Activation of chloride secretion via proteinase-activated receptor 2 in a human eccrine sweat gland cell line – NCL-SG3


Dr Roland Lang, Department of Dermatology, University Hospital Salzburg, Paracelsus Medical University Salzburg, Muellner Hauptstrasse 48, A-5020 Salzburg, Austria, Tel.: +43 662 4482 3075, Fax: +43 662 4482 3053, e-mail:


Abstract:  Proteinase-activated receptor 2 (PAR-2) has been shown to elicit secretion in a variety of secretory epithelial cells by the transepithelial movement of chloride ions across the apical membrane. However, it is not known whether these receptors are present and/or functional in the secretory epithelial cells of the human eccrine sweat gland. To investigate this possibility mRNA analysis, Ca2+ microspectrofluorimetry and the short circuit current (Isc) technique were used to quantify electrolyte transport in a cell line (NCL-SG3) derived from human eccrine sweat gland secretory epithelia. The results provided molecular and functional evidence of the presence of PAR-2 receptors in the NCL-SG3 cells and show that these receptors can activate transepithelial Cl secretion possibly via Ca2+-activated Cl channels.