Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA component, TERC

Authors


Aloysius J. Klingelhutz, 2202 MERF, 375 Newton Road, Iowa City, 52242 IA, USA, Tel.: 319 335 7788, Fax: 319 353 4556, e-mail: al-klingelhutz@uiowa.edu

Abstract

Please cite this paper as: Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA component, TERC. Experimental Dermatology 2010; 19: 279–288.

Abstract:  Dyskeratosis congenita (DC) is characterized by the triad of reticulate skin pigmentation, nail dystrophy and leukoplakia. Epidermal atrophy, hair growth defects, bone marrow failure and increased risk of cancer are also common in DC patients. DC is caused by mutations in genes encoding for telomerase complex factors. Although there is an association of epidermal abnormalities with DC, epidermal cells from DC donors have not been previously characterized. We have isolated skin keratinocytes from affected members of a family with an autosomal dominant form of DC that is caused by a mutation in the RNA component of telomerase, TERC. Here, we demonstrate that, similar to DC fibroblasts from these donors, DC keratinocytes have short telomeres and a short lifespan. DC keratinocytes also exhibited impaired colony forming efficiency (CFE) and migration capacity. Exogenous expression of the reverse transcriptase (RT) component of telomerase, TERT, activated telomerase levels to half that of TERT expressing normal cells and maintained telomeres at a short length with concomitant extension of lifespan. Unlike fibroblasts, transduction of human papillomavirus type 16 E6/E7 genes into DC keratinocytes activated telomerase to half that of E6/E7 expressing normal cells, and robust proliferation was observed. While expression of TERC has no measurable effect on telomerase in fibroblasts, expression of TERC in keratinocytes upregulated telomerase activity and, rarely, allowed rescue of proliferative defects. Our results point to important differences between DC fibroblasts and keratinocytes and show, for the first time, that expression of TERC can increase the lifespan of primary human epithelial cells.

Introduction

Telomeres consist of hexameric tandem repeats (TTAGGG) of DNA located at the chromosome ends and are necessary for maintaining chromosome integrity, function and replication (1,2). In the absence of a mechanism to maintain them, telomeres normally shorten with each cell division and with ageing (3). Telomeres can be maintained by the telomerase complex, which minimally consists of an RNA component called TERC (hTERC or mTERC for the human or mouse gene, respectively; also often referred to as TR or TER) that acts as a template for a RT component, TERT, that catalyses the addition of telomere repeats to the telomere ends (4,5). Other protein components such as dyskerin, a nucleolar ribonucleoprotein with pseudouridinylation properties, bind to the telomerase complex to stabilize and affect its localization and regulate its activity (6). Telomerase is active in human germline cells and most cancers (7,8). Most normal human somatic cells express TERC but have low to undetectable levels of TERT and low levels of telomerase (8). Epidermal skin cells and haematopoietic cells have some telomerase activity (9–11).

Dyskeratosis congenita (DC) is a premature ageing syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy and oral leukoplakia, and most patients die as a result of bone marrow failure (12–15). A variety of other somatic abnormalities normally seen in aged individuals have also been reported in DC, including hair loss, epidermal atrophy, grey hair, osteoporosis and pulmonary and hepatic fibrosis (15–17). DC patients also display an increased risk for malignancy (18). DC is caused by mutations in genes involved in telomerase or telomere function (16,17). The DKC gene involved in the X-linked version of the disease encodes dyskerin, which interacts with TERC and is required for its accumulation (6,19–22). Autosomal dominant forms of DC (AD DC), can be caused by mutations in TERC or TERT (23–25). In these families, one allele of TERC or TERT is generally made non-functional by point mutation or deletion and this leads to haploinsufficiency for telomerase and short telomeres. Other mutations in telomerase component genes and telomere binding proteins have also recently been detected in DC patients (17).

Previous studies from our laboratory have shown that fibroblasts from DC donors from a family with a deletion of a single alleles of TERC have very short telomeres, a short lifespan and proliferative defects (26). Despite the clear association of epidermal and mucosal abnormalities with DC, no studies have been performed using epidermal cells from DC donors. Fibroblasts and epidermal cells are known to differ in telomerase regulation and proliferation. For example, keratinocytes have some active telomerase, because of expression of TERT, but telomerase in fibroblasts is not detectable (27). Exogenous expression of TERT has been shown to immortalize both fibroblasts and keratinocytes, when grown in the right growth conditions (28–30). Expression of TERC alone has no detectable effects on telomerase or proliferation in primary fibroblasts (26,31) and there have been no reports on the effects of TERC in keratinocytes or other primary epithelial cells. Here, we have isolated and characterized skin keratinocytes from affected donors of a TERC deficient DC family. We hypothesized that the DC keratinocytes would differ from DC fibroblasts because of intrinsic differences in telomerase biology between keratinocytes and fibroblasts. We found that, like DC fibroblasts, DC keratinocytes exhibited both proliferative and functional defects. Exogenous expression of TERT alone activated telomerase and extended the lifespan of DC keratinocytes. Unlike DC fibroblasts, exogenous expression of HPV-16 E6/E7 or the RNA component, TERC, activated telomerase in DC keratinocytes and provided some measure of rescue of proliferative defects. To our knowledge, this is the first characterization of skin keratinocytes from DC patients and the first demonstration that TERC alone can increase telomerase activity and the lifespan of primary human epithelial cells.

Methods

Cell isolation and culture

Keratinocytes were isolated as previously described from punch biopsies of the skin from the buttocks region of AD DC and normal donors (26,32). The punch biopsies were from third and a second generation adult DC subjects (DC-HSK-1 and DC-HSK-4). The studies were approved by the University of Iowa Institutional Review Board for human studies and conformed to protocols outlined by the Declaration of Helsinki. Donors gave their written, informed consent for collection of tissue. Normal sex and age (within 1 year) matched biopsies were used as controls (referred to as N-HSK-1 and N-HSK-2). Briefly, the tissue was incubated overnight in dispase and on the following day, the epidermal layer was treated with trypsin to dissociate epithelial cells. The cells were plated on irradiated J2 3T3 feeders in E-media as described (33,34). When 80% confluent, the cells were passed 1:4 [approximately 2 population doublings (pd) per passage]. Passaging of cells was performed by differentially removing feeder fibroblasts with a low per cent trypsin/EDTA followed by complete trypsinization of remaining epithelial cells. For certain procedures, such as isolation of DNA, epithelial cells were passaged into keratinocyte serum-free media (KSFM) for two passages to remove feeders before collection of cells.

CFE and in vitro scratch assay

For the CFE assay, feeder cells were differentially removed with low per cent trypsin and washes with phosphate-buffered saline. Epithelial cells were collected with further trypsinization and counted. The cells were plated at 200 cells per 60 mm plate in triplicate. Irradiated feeders (0.5 million/plate) were added to each plate and cells were media-changed every 3rd day. On the 12th day, colonies were stained by first fixing in 95% ethanol, followed by staining with 1% methylene blue in 50% ethanol, followed by a water rinse. Only those colonies that were 50 cells or more were counted. Per cent CFE was calculated as the number of colonies divided by the total number of cells plated times 100. Average and standard error were calculated from triplicate samples.

For the scratch wound migration assay, cells were grown to 85–90% confluency in E-media and plates were marked so that a grid on the bottom of the plate could be used to keep track of location. The cultures were then scratched with four scrapes using a 200 μl yellow pipette tip. Images were captured for each cell type immediately after the scrapes were performed (0 h) followed by monitoring of scrape closure by taking digital photographs of the same locations over a time course. The width of each scrape was measured for each of the four images at different time points and the average per cent closure (as compared to 0 h) was calculated along with standard error of the mean.

Retroviral constructs, virus generation and transduction

For expression of HPV-16 E6E7, cells were transduced with HPV-16 E6/E7 LXSN replication defective retrovirus and selected in G418. The HPV-16 E6/E7 LXSN construct has been previously described (35). The TERT-cherry construct was generated by cloning full-length hTERT into a pHIV7 vector (36). Expression of TERT was driven by a spleen focus forming virus promoter (SF promoter). Expression of mCherry was accomplished through an IRES (Internal Ribosome Entry Site). The TERC-Cherry vector was generated by cloning the TERC gene, plus the 3′ genomic region, driven by a U3 promoter followed by Cherry driven by an SF promoter. Virus was produced in 293 packaging lines by the University of Iowa Gene Vector Core using established protocols (36). Cells were infected at 5 MOI (Multiplicity of Infection). For neomycin vectors, cells were selected in G418. For the mCherry vectors, cells were sorted by FACS (Fluorescence Activated Cell Sorting) by gating for cherry fluorescence.

Southern blotting, telomerase assay and quantitative RT-PCR

Southern blotting was performed as previously described using 3 μg of genomic DNA digested with RsaI/HinfI (37). Digested DNA was separated on a 0.7% agarose gel, transferred to Hybond N membrane (Amersham, Little Chalfont, UK), and fixed with UV by a Stratalinker (Stratagene, La Jolla, CA, USA). Telomere signal was detected using a DIG (Digoxigenin) labelled telomere sequence probe followed by chemiluminescence detection methodology from Roche according to the manufacturer’s protocol (Roche, Indianapolis, IN, USA). Telomere length was calculated by finding the midpoint of the telomere signal and comparing to DIG labelled size standards run on the same gel.

The telomerase assays were performed as previously described using a quantitative PCR methodology (26). Lysate from one thousand cells was used per assay and each assay was performed in quadruplicate and average values were calculated along with standard error. Results were calibrated to a standard curve and made relative to the telomerase activity detected in normal skin keratinocytes for comparison.

Quantitative reverse transcriptase PCR (q-RT-PCR) was performed as described (26). Briefly, total RNA was extracted from cells grown for one passage without feeders using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions and purified with RNeasy Mini Columns (Qiagen, Valencia, CA, USA). RNA was reverse transcribed using a Retroscript kit (Ambion, Austin, TX, USA) with random decamers as recommended by the manufacturer. Quantitative PCR was performed in triplicate using the ABI PRISM 7900 Sequence Detection System with standard cycling in SYBR-Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) with the following primers: TERC-F, 5′-TCTAACCCTAACTGAGAAGGGCGTAG-3′; TERC-R, 5′-GTTTGCTCTAGAATGAACGGTGGAAG-3′; TERT-v1-F, 5′-TGTACTTTGTCAAGGTGGATGTGA-3′; TERT-v1-R, 5′-GCTGGAGGTCTGTCAAGGTAGAG-3′; GAPDH-F, 5′-AAGGTCATCCATGACAACTTTG-3′; GAPDH-R, 5′-GTAGAGGCAGGGATGATGTTCT-3′. Expression levels were normalized to GAPDH and made relative to normal untransduced N-HSK-1 cells.

Results

We isolated and cultured skin keratinocytes from skin punch biopsies of third and second generation DC individuals (DC-HSK-1 and DC-HSK-4, respectively) and age/sex-matched controls (N-HSK-1 and N-HSK-2) for our analysis (Table 1). Affected members of the DC family have a mutation in the TERC gene that results in deletion of the last 74 bases of the TERC RNA (23). Initially, DC-HSK proliferated at rates similar to their normal counterparts, but the lifespan of the DC-HSK cells was shorter than that of the normal controls (Fig. 1a and Table 1). Using a CFE assay, we found that keratinocytes from both the second and third generation donors had significantly less CFE than normal counterparts (Fig. 1b and Table 1). Not only were there fewer colonies in the DC plates but also the colonies were smaller, overall (data not shown). The least CFE was noted for cells from the third generation DC-HSK-1 cells, while the best CFE was noted for N-HSK-1 (2% and 7%, respectively). We also assessed migration ability using a scratch wound assay. For this assay, we chose a time point of 18 h for comparison since normal keratinocytes exhibited 60–80% closure at this time but were closed completely at 24 h (example photographs of the scratch assay at 0 and 18 h are shown in Fig. 2a). DC-HSKs exhibited defects in migration and were only 30–50% closed at 18 h (Fig. 2b and Table 1). As with CFE, the cells from the third generation donor (DC-HSK-1) exhibited the greatest defect and the normal N-HSK-1 cells had the most rapid closure. Flow cytometric analysis of cell cycle profiles of subconfluent cells indicated that the differences in wound closure capacity between DC and normal keratinocytes were not caused by an overall block in cell cycle or proliferation (data not shown). Overall, our results indicate that DC-HSKs are defective in their ability to proliferate and in their ability to migrate in a scratch wound assay.

Table 1.   Characteristics of primary skin keratinocytes from dyskeratosis congenita donors compared with normal donors
Primary keratinocyte strainDonor characteristics1Maximum lifespan in culture (pd)2% Colony forming efficiency3% Wound closure (18 h)4Average telomere length (kb)5
  1. 1Keratinocytes were obtained from either normal or affected dyskeratosis congenita (DC) donors. The sex (M or F), age of the donor and generation (if applicable) are shown.

  2. 2Maximum in vitro lifespan in population doublings (pd) (see Fig. 1a).

  3. 3Colony forming efficiency on irradiated feeders (see Fig. 1b).

  4. 4Per cent scratch wound closure 18 h compared with 0 h (see Fig. 2).

  5. 5Average telomere length estimated from Southern blot analysis (see Fig. 3a).

N-HSK-1Normal/M/40 years326.2 ± 1.080.4 ± 8.210.1
N-HSK-2Normal/F/22 years324.0 ± 0.365.0 ± 12.19.6
DC-HSK-1DC/F/21 years/3rd gen.181.5 ± 0.537.1 ± 5.95.9
DC-HSK-4DC/M/40 years/2nd gen.223.5 ± 0.643.4 ± 7.66.2
Figure 1.

 Keratinocytes from dyskeratosis congenita (DC) donors have proliferative defects as compared to keratinocytes from age- and sex-matched normal donors. (a) Population doublings of skin keratinocytes grown with irradiated feeder fibroblasts. Cells were passaged as described in Materials and Methods. DC-HSK-1 and DC-HSK-4 are keratinocytes from third and second generation affect DC subjects respectively. N-HSK-1 and N-HSK-2 are sex- and age-matched keratinocytes from a normal donors. DC-HSK-1 is age and sex matched with N-HSK-2 and DC-HSK-4 is age and sex matched with N-HSK-1. (b). Colony forming efficiency (CFE) of cells grown on irradiated feeder fibroblasts. CFE assays were performed as described in the Methods. Error bars represent standard error based on three replicates.

Figure 2.

 Primary dyskeratosis congenita keratinocytes exhibit slower closure of scratch wounds in vitro. (a) Confluent cultures were scraped with a pipette tip and followed over time to assess closure as described in Methods. Representative photos of 0- and 18-h time point are shown. (b) Average percent scratch wound closure at 18 h as compared to 0 h time point. Error bars represent standard error of four replicate scratches.

Telomere length in the primary DC and normal keratinocytes was assessed by Southern blot analysis using a telomere specific probe on digested genomic DNA. This analysis revealed that DC keratinocytes had significantly shorter telomeres (approximately 6 kb) than normal keratinocytes from age- and sex-matched normal donors (range: 9–11 kb) (Fig. 3a and Table 1). Telomeres in keratinocytes from the two DC donors were of similar length, although it should be noted that the third generation donor was younger (21-year old) than the second generation donor (41-year old).

Figure 3.

 Telomere length analysis of primary and transduced keratinocytes from dyskeratosis congenita (DC) and normal donors. Southern analysis was performed as described in the Methods. For transduced cells, EP, early passage (with 10 pd after transduction); LP, later passage (c. 30–40 pd post-transduction). Calculated average telomere lengths are summarized in Table 2. (a) Primary DC and normal keratinocytes. Sex- and age-matched controls are shown in pairs. Lane 1, size marker; Lane 2, N-HSK-2; Lane 3, DC-HSK-1; Lane 4, N-HSK-1; Lane 5, DC-HSK-4; Lane 6, size marker. (b) Telomere length in TERT-transduced keratinocytes with time in culture. Lane 1, size marker; Lane 2, primary N-HSK-1; Lane 3, TERT-transduced N-HSK-1; Lane 4, TERT-transduced N-HSK-1 at later passage; Lane 5, primary DC-HSK-1; Lane 6, TERT-transduced DC-HSK-1; Lane 7, TERT-transduced DC-HSK-1 at later passage. (c) Telomere length analysis of E6/E7-transduced keratinocytes. Lane 1, size marker; Lane 2, primary N-HSK-1; Lane 3, E6/E7-transduced N-HSK-1; Lane 4, E6/E7-transduced N-HSK-1 at later passage; Lane 5, primary DC-HSK-1; E6/E7-transduced DC-HSK-1; Lane 7, E6/E7-transduced DC-HSK-1 at later passage; Lane 8, size marker. (d) Telomere length in TERC-transduced DC keratinocytes. Lane 1, size marker; Lane 2, primary N-HSK-1; Lane 3, primary DC-HSK-1; Lane 4, TERC-transduced DC-HSK-1; TERC-transduced DC-HSK-1 at later passage.

Because DC-HSK-1 and N-HSK-1 represented the two extremes for CFE and scratch wound closure, we decided to further characterize these cells for telomerase activity and immortalization studies. Telomerase activity, as measured by a quantitative telomere repeat amplification protocol (TRAP) assay, was barely detectable in DC keratinocytes and was approximately half the level of that observed in primary untransduced normal cells (Table 2). Similar findings have been reported in DC lymphocytes compared with age-matched controls and would be expected because the DC cells have one wild type and one defective copy of TERC (38). As measured by q-RT-PCR, levels of TERC transcript in the DC keratinocytes were lower than in normal keratinocytes and, in addition, levels of TERT transcript were lower in DC cells, both of which could account for the lower levels of telomerase in untransduced DC cells.

Table 2.   Telomerase and telomere length in primary and transduced normal and dyskeratosis congenita keratinocytes
Cell nameRelative telomerase activity1Relative TERT transcript levels2Relative TERC transcript levels3Average telomere length (kb)4Average telomere length (later passage) (kb)5
  1. 1Relative telomerase activity as measured by a quantitative real-time TRAP assay. The shown activity is relative to normal untransduced keratinocytes (N-HSK-1).

  2. 2Relative TERT transcript as measured by quantitative reverse transcriptase PCR (q-RT-PCR). The shown levels were normalized to GAPDH and are relative to untransduced N-HSK-1.

  3. 3Relative levels of the RNA component, TERC, as measured by q-RT-PCR. Shown levels were normalized to GAPDH and are relative to untransduced N-HSK-1.

  4. 4Average telomere length of various primary or transduced cells within 5–10 pd after transduction as measured by Southern blotting (summarized from blots shown in Fig. 3).

  5. 5Average telomere length of various transduced cells approximately 30–40 pd after transduction (i.e. latter passage) as measured by Southern blotting (summarized from blots shown in Fig. 3).

N-HSK-11.0 ± 0.41.0 ± 0.21.0 ± 0.210.1ND
DC-HSK-10.5 ± 0.10.5 ± 0.40.3 ± 0.15.9ND
TERT-N-HSK-1444.0 ± 115.03.2 × 10± 2.0 × 1040.7 ± 0.110.010.7
TERT-DC-HSK-1290.0 ± 31.06.5 × 10± 3.0 × 1030.6 ± 0.14.44.3
E6/E7-N-HSK-117.8 ± 7.55.8 ± 1.12.8 ± 0.48.66.6
E6/E7-DC-HSK-16.7 ± 0.54.1 ± 0.51.4 ± 0.36.03.2
TERC-DC-HSK-14.2 ± 0.20.3 ± 0.234.9 ± 3.33.83.6

Because the DC keratinocytes had lower levels of TERC, we were interested in determining whether they were defective in their ability to be immortalized by exogenous expression of the RT component of telomerase, TERT, which has been shown to robustly activate telomerase and immortalize various cell types. Exogenous expression of TERT using a retroviral vector greatly increased telomerase (more than 200-fold) in both DC and normal keratinocytes (Table 2). Like the untransduced cells, the TERT-transduced DC cells exhibited approximately half the telomerase activity of TERT-transduced normal cells (Table 2). As expected, TERT transcript was highly increased in the TERT-transduced normal and DC cells, although, for unknown reasons, the TERT-transduced DC cells had much lower levels of TERT transcript than TERT-transduced normal cells. Exogenous expression of TERT slightly increased levels of TERC transcript in DC cells but not in normal cells, indicating that the greatly increased levels of telomerase activity in both cell types was mostly because of high levels of TERT expression. Both TERT-transduced DC and normal keratinocytes had an extended lifespan of more than five times that of untransduced cells and no apparent crisis was observed (Table 3). The cells continue to proliferate and are apparently immortal. Although telomere length was not significantly elongated in TERT expressing DC or normal cells, it was maintained at a stable length with passaging in culture (Fig. 3b and Table 2). These results indicate that TERT expression in DC keratinocytes upregulates telomerase to a level that is sufficient for maintenance but not necessarily enough to allow for significant lengthening of telomeres. A similar finding was previously observed for TERT expressing DC skin fibroblasts (26). Expression of exogenous TERT increased CFE in both normal and DC keratinocytes, although the TERT-transduced DC keratinocytes exhibited consistently lower CFE than TERT-transduced normal keratinocytes, perhaps because of lower telomerase activity or shorter telomere length (Tables 2 and 3). Both TERT-transduced DC and normal keratinocytes had a similar rate of scratch wound closure compared with that of untransduced normal cells, indicating rescue of this defect by exogenous expression of TERT in DC cells (Table 3).

Table 3.   Characteristics of transduced normal and dyskeratosis congenita keratinocytes
Cell nameMaximum lifespan (pd)1% Colony forming efficiency2% Wound closure (18 h)3 Karyotype4
  1. 1Lifespan in cell culture in population doublings (pd). Maximum lifespan shown for untransduced cells. For the transduced cells, a “>“ symbol signifies continuous growth.

  2. 2Per cent colony forming efficiency on irradiated feeders.

  3. 3Per cent scratch wound closure at 18 h as compared to 0 h.

  4. 4Cytogenetic analyses were performed within 20–30 pd post-transduction. The number in bracket represents the number of metaphases out of 20 that exhibited the noted karyotype.

N-HSK-1327.0 ± 0.487.2 ± 8.646, XY[20] (normal)
DC-HSK-1182.1 ± 0.321.3 ± 12.746, XX[20] (normal)
TERT-N-HSK-1>9020.8 ± 1.6100.0 ± 046, XY[20] (normal)
TERT-DC-HSK-1>9011.7 ± 2.190.3 ± 7.946, XX[20] (normal)
E6/E7-N-HSK-1>9039.3 ± 4.079.4 ± 7.946, XY, i(8)(q10)[18]/47, XY,+6[2]
E6/E7-DC-HSK-1>9045.2 ± 6.344.0 ± 1.748, XX, i(8)(q10),+11,+20[20]
TERC-DC-HSK-1>6016.3 ± 0.351.7 ± 1.646, XX[20] (normal)

We next wanted to assess whether the DC keratinocytes could be immortalized by exogenous expression of human papillomavirus type 16 (HPV-16) E6 and E7 which, together, are known to effectively immortalize many human epithelial cell types, including human keratinocytes (39). The E6 and E7 proteins from high-risk HPV types such as HPV-16 inactivate p53 and pRb pathways, respectively, but are also known to activate endogenous telomerase through upregulation of TERT (40–42). Transduction of DC and normal keratinocytes with HPV-16 E6/E7 retrovirus resulted in upregulation of telomerase, as expected, and the E6/E7-transduced DC keratinocytes exhibited approximately half that of E6/E7-transduced normal cells, (Table 2). Not surprisingly, TERT transcript was higher in E6/E7-transduced cells but was nowhere near the levels observed for TERT-transduced cells (Table 2). Interestingly, levels of TERC transcript were also up in E6/E7-transduced DC and normal keratinocytes, indicating that immortalization of cells by E6/E7 may result in activation of telomerase through upregulation of both endogenous TERT and TERC. Expression of E6/E7 resulted in a greatly extended lifespan in both DC and normal keratinocytes (greater than five times the lifespan of untransduced cells) with no apparent crisis or slow down in growth (Table 3). At early passage, the E6/E7-transduced DC and normal cells had telomere lengths that were comparable with untransduced cells. Despite upregulation of telomerase, both E6/E7-transduced DC and normal keratinocytes exhibited significant telomere shortening with passaging in culture (Fig. 3c and Table 2). E6/E7 expression greatly increased CFE in both DC and normal keratinocytes (Table 3). Although E6/E7 increased scratch wound closure rate of DC keratinocytes, it did not approach the rate of closure observed for either non-transduced or E6/E7 expressing normal keratinocytes, suggesting that E6/E7 in DC cells could not completely correct the scratch wound defect (Table 3).

Our previous studies demonstrated that exogenous expression of the RNA component of telomerase, TERC, had no effect on proliferation or telomerase activity in DC fibroblasts (26). Modest two- to threefold increases in telomerase activity were observed upon transduction of primary DC and normal keratinocytes with TERC (data not shown). While we did not observe robust telomerase activation by TERC in DC keratinocytes, a subpopulation emerged from TERC-transduced DC keratinocytes with telomerase activity that was four- to fivefold greater than untransduced DC keratinocytes (Table 2). As expected, a concomitant increase in TERC transcript levels was observed in the TERC-transduced cells. However, there was no detectable increase in TERT levels in these cells, indicating that the increase in telomerase was because of exogenously expressed TERC (Table 2). The TERC-transduced DC cells did not exhibit any apparent extension of telomere length beyond that of untransduced cells (in fact they were shorter) but maintained telomeres at a very short length of approximately 3.6 kb (Table 2 and Fig. 3d). Even with telomere shortening, the TERC-transduced cells had a greatly extended lifespan of >50 pd beyond that observed for untransduced DC cells (Table 3). As mentioned above, exogenous expression of TERC slightly increased telomerase in normal keratinocytes, but we did not observe significant extension of lifespan in these cells (data not shown). However, in an independent study, we have noted upregulation of telomerase and slight extension of lifespan by TERC transduction in neonatal foreskin keratinocytes (A.J. Klingelhutz, unpublished data). Further characterization of the TERC-transduced DC keratinocytes demonstrated increased CFE and some measure of amelioration of the scratch wound closure defect as compared with untransduced DC cells (Table 3).

Finally, to assess whether the short telomeres in DC cells were associated with genetic instability upon immortalizaton, we examined the various transduced cells by Giemsa banding. Karyotypes were performed between 15 and 25 pd post-transduction (Table 3). Both the TERT-transduced DC and normal keratinocytes exhibited completely normal karyotypes. The E6/E7-transduced DC and normal cells, on the other hand, exhibited some cytogenetic abnormalities, but the E6/E7-transduced DC cells did not have extensive gross genetic alterations as might be expected to result from having such short telomere length. However, we cannot rule out the possibility that genetically stable subclones emerged after a period of genetic instability at earlier passage. Interestingly, the TERC-transduced DC keratinocytes with an extended lifespan had no apparent cytogenetic abnormalities, indicating the expression of TERC alone was sufficient to maintain telomeres and genetic stability in this cell population.

Discussion

The studies presented here demonstrate that keratinocytes from DC subjects have short telomeres, and in addition, shortened lifespan and measurable defects in colony forming ability and migration capacity. Similar to our studies with DC fibroblasts, immortalization of DC keratinocytes by exogenous expression of TERT caused significant upregulation of telomerase, and maintenance of telomeres. Unlike fibroblasts, expression of HPV E6/E7 could upregulate telomerase in keratinocytes. While expression of TERC had no observable effects in fibroblasts, our studies indicate that TERC expression upregulated telomerase in keratinocytes and allowed extension of lifespan in a subpopulation of DC keratinocytes. This is, to our knowledge, the first report of TERC having any effect on the proliferative capacity of primary human cells. Overall, our results provide further understanding of the potential mechanisms that may be behind the epidermal defects associated with shortened telomeres in DC. They also corroborate significant differences between fibroblasts and keratinocytes with regard to telomerase biology.

How telomerase and telomere shortening affect the ageing of the epidermis is not clear. It has been argued that epidermal stem cells do not age and that levels of telomerase in keratinocytes are sufficient to maintain telomeres throughout life (27,43). The epidermis is one of the few regenerative tissues that expresses telomerase activity; telomerase has been detected in basal epithelial cells and in the bulb-containing region of hair follicles (10,44,45). Studies have indicated, however, that telomere length decreases in epidermal cells with age (46,47). Significant telomere shortening has been observed in cultured skin autografts of patients with burns (48). Chronic ischaemia in skin injuries and delayed wound healing are a feature of ageing, and it has been speculated that this might be related to telomere shortening or downregulation of telomerase (49). While telomerase knockout mice have provided insight into the role of telomere shortening in ageing, mice and humans are known to be different with regard to telomere length and telomerase regulation (16). DC provides a unique model system for studying how telomerase dysfunction and/or telomere shortening may be involved in the ageing of human epidermis. The observation that DC individuals exhibit numerous skin defects, including poor wound healing (17,50) and epidermal atrophy (15) indicates that telomere length does play a role in skin biology and our in vitro studies using DC keratinocytes support this hypothesis. It is noteworthy that keratinocytes isolated from the third generation DC subject (DC-HSK-1) had the most profound proliferative and migratory defects, and similarly had the most severe phenotype and lowest CD34+ haematopoietic stem cell counts (51). One possibility is that keratinocyte stem cells in DC patients have insufficient telomerase, and telomere associated senescence contributes to epidermal problems in DC patients. Similar mechanisms could be involved in normal ageing of skin. However, other factors, including mesenchymal support cells in the skin, are also likely to play a role and these need to be taken into account when discussing models of skin ageing (27). It is unknown whether the low levels of constitutive telomerase observed in keratinocyte stem cells contributes significantly to telomere maintenance and homeostasis in the adult epidermis. This possibility might need to be considered as a potential cause for side-effects in treatment of cancers with telomerase inhibitors (52,53).

Our studies suggest that exogenous expression of TERT or TERC may provide a means to correct certain proliferative problems in the epidermis. Studies using rabbits and mice have indicated that overexpression of TERT can increase wound healing capacity (54,55). The published finding that exogenous expression of TERT, even in TERC knockout mice, can lead to mobilization of epidermal stem cells would indicate that TERT has an important role in keratinocyte biology independent of telomere elongation (56–58). In this regard, TERT has been implicated in being involved in inhibition of apoptosis and resistance to DNA damaging agents through widespread changes in gene expression (56,57,59–64). Some of these observed effects may be as a result of gross overexpression of TERT, which could impact other cell processes. That exogenous expression of TERC can upregulate endogenous telomerase, albeit slightly, in primary keratinocytes and extend their lifespan is of significant interest. Recently, it was reported that exogenous expression of TERC could increase telomerase in primary T cells and EBV-transformed lymphoblastoid cell lines (LCL) derived from DC patients (65). In EBV-transformed LCLs, TERC expression was shown to increase proliferative capacity, as well. Extension of lifespan by TERC alone has not been previously demonstrated for primary human cells, likely because most studies have been done with cells such as fibroblasts, which have detectable levels of TERC but nearly undetectable levels of TERT. Keratinocytes have low levels of TERT, and our results would suggest that a subpopulation of keratinocytes can survive by expression TERC alone such that they have increased telomerase and an extended lifespan. It is unknown whether the few immortal cells that emerged from transduced DC keratinocytes were progenitor-like cells that already had sufficient levels of TERT to maintain telomeres. Our results would suggest that both TERT and TERC are rate limiting for telomerase activity in human keratinocytes. Such a mechanism has been proposed previously for other cell types (66). While exogenous expression of either or both of these genes might upregulate telomerase and maintain telomeres in keratinocytes, any attempts to introduce TERT or TERC into tissues for treatment of disease would need to take into account the fact that telomerase activation could result in malignant transformation.

Short telomeres have been implicated in the development of genetic instability and concomitant malignant transformation, which has been suggested as a potential mechanism to explain the increased risk of malignancy in DC patients (17,67). Interestingly, we did not observe a large number of chromosome alterations in E6/E7-transduced DC cells even though they had very short telomeres. The E6/E7 cells continued to exhibit telomere shortening and continued to proliferate, most likely caused by bypass of the normal DNA damage response through abrogation of p53 and pRb pathways by E6 and E7. With further telomere shortening, these cells may eventually exhibit more profound genetic instability or genetic catastrophe and cell death. However, we and others have shown that E6/E7 expressing keratinocytes eventually stabilize and sometimes even elongate telomeres with time in culture (37,68). It is interesting to note that no large-scale cytogenetic alterations were observed in the TERC-expressing DC-HSK-1 clones that emerged in culture. Although other unapparent transforming events may have occurred in these cells, this result would suggest that exogenous expression of TERC maintains telomeres at sufficient length to prevent end-to-end fusions and gross genetic instability.

In summary, our studies implicate a causal role of telomere shortening in the ageing of skin keratinocytes and the epidermal pathologies observed in DC. While similar to DC fibroblasts in their proliferative defects and ability to be immortalized by TERT, DC keratinocytes differ from fibroblasts in their ability to activate telomerase upon expression of E6/E7 or TERC. Our studies suggest that exogenous expression of TERC can increase telomerase and lifespan in primary cells such as keratinocytes that naturally express low levels of endogenous telomerase. Overall, our results may have implications for understanding the role of telomere shortening in the ageing of human skin and for developing novel strategies for treatment of epidermal problems associated with ageing.

Acknowledgements

The authors wish to thank associates of the Gene Transfer Vector Core Facility of the University of Iowa Center for Gene Therapy of Cystic Fibrosis and Other Genetic Diseases, supported by NIH/NIDDK P30 DK 54759 for their assistance with viral vector production. We also thank the University of Iowa DNA Facility and Flow Cytometry Cores for q-PCR and FACs analysis. We are also grateful to Kathy Collins for the hTERC construct, Robert Weinberg for the hTERT construct and Jiing-Kuan Yee for the pHIV7 vector. We thank Kimberly Lee for technical assistance. Cytogenetic analysis was performed at the University of Iowa Cytogenetics Facility. The work in the laboratory of PML was supported by a grant from the Canadian Institutes of Health Research (MOP38075). The work in the laboratories of FDG and AJK was supported by a grant from the National Institutes of Health (R01 AG0227388).

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