Proteomic profiling reveals a catalogue of new candidate proteins for human skin aging


Martin Laimer, MD, Division of Molecular Dermatology, Department of Dermatology, Paracelsus Private Medical University Salzburg, Muellner Hauptstrasse 48, 5020 Salzburg, Austria, Tel.: +43 662 4482 3001, Fax: +43 662 4482 3003, e-mail:


Abstract:  Studies of skin aging are usually performed at the genomic level by investigating differentially regulated genes identified through subtractive hybridization or microarray analyses. In contrast, relatively few studies have investigated changes in protein expression of aged skin using proteomic profiling by two-dimensional (2-D) gel electrophoresis and mass spectrometry, although this approach at the protein level is suggested to reflect more accurately the aging phenotype. We undertook such a proteomic analysis of intrinsic human skin aging by quantifying proteins extracted and fluorescently labeled from sun-protected human foreskin samples pooled from ‘young’ and ‘old’ men. In addition, we analyzed these candidate gene products by 1-D and 2-D western blotting to obtain corroborative protein expression data, and by both real-time PCR (RT-PCR) and microarray analyses to confirm expression at the mRNA level. We discovered 30 putative proteins for skin aging, including previously unrecognized, post-translationally regulated candidates such as phosphatidyl-ethanolamine binding protein (PEBP) and carbonic anhydrase 1 (CA1).