These two authors contributed equally to this work.
Centella asiatica extracts modulate hydrogen peroxide-induced senescence in human dermal fibroblasts
Article first published online: 17 NOV 2011
© 2011 John Wiley & Sons A/S
Volume 20, Issue 12, pages 998–1003, December 2011
How to Cite
Kim, Y. J., Cha, H. J., Nam, K. H., Yoon, Y., Lee, H. and An, S. (2011), Centella asiatica extracts modulate hydrogen peroxide-induced senescence in human dermal fibroblasts. Experimental Dermatology, 20: 998–1003. doi: 10.1111/j.1600-0625.2011.01388.x
- Issue published online: 17 NOV 2011
- Article first published online: 17 NOV 2011
- Accepted manuscript online: 11 OCT 2011 01:51AM EST
- Accepted for publication 29 September 2011
- Centella asiatica;
- dermal fibroblasts;
- gene expression;
- hydrogen peroxide;
Abstract: Centella asiatica (C. asiatica) is a pharmacological plant in South Asia. It has been demonstrated that C. asiatica extracts containing various pentacyclic triterpenes exert healing effects, especially wound healing and collagen synthesis in skin. However, there are few studies on the effect of C. asiatica extracts on stress-induced premature senescence (SIPS). To determine whether H2O2-induced senescence is affected by C. asiatica extracts, we performed senescence analysis on cultured human dermal fibroblasts (HDFs). We also analysed whole gene expression level using microarrays and showed that 39 mRNAs are differentially expressed in H2O2-induced HDFs with and without treatment with C. asiatica extracts. These genes regulate apoptosis, gene silencing, cell growth, transcription, senescence, DNA replication and the spindle checkpoint. Differential expression of FOXM1, E2F2, MCM2, GDF15 and BHLHB2 was confirmed using semi-quantitative PCR. In addition, C. asiatica extracts rescued the H2O2-induced repression of replication in HDFs. Therefore, the findings presented here suggest that C. asiatica extracts might regulate SIPS by preventing repression of DNA replication and mitosis-related gene expression.