Phalloidin-induced morphological and functional changes of rat liver

Authors

  • Roel J. Vonk,

    1. Centre de Recherche Pédiatrique, Hôpital Sainte-Justine and Département de Nutrition, Université de Montréal, Montréal, Québec, Canada
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  • Ibrahim M. Yousef,

    Corresponding author
    1. Centre de Recherche Pédiatrique, Hôpital Sainte-Justine and Département de Nutrition, Université de Montréal, Montréal, Québec, Canada
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  • J. P. Corriveau,

    1. Centre de Recherche Pédiatrique, Hôpital Sainte-Justine and Département de Nutrition, Université de Montréal, Montréal, Québec, Canada
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  • Beatriz Tuchweber

    1. Centre de Recherche Pédiatrique, Hôpital Sainte-Justine and Département de Nutrition, Université de Montréal, Montréal, Québec, Canada
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Centre de Recherche Pédiatrique Hôpital Sainte-Justine 3175, Chemin de la Câte Ste-Catherine Montréal, Québec H3T 1C5 Canada

Abstract

ABSTRACT— The hepatotoxic effect of phalloidin, a mushroom poison, was examined in whole liver and in isolated liver cell plasma membranes. By electron microscopy, a thickening of the pericanalicular microfilamentous network was noted. Bile canalicular enriched plasma membrane fractions of livers from phalloidin-treated animals contained increased amounts of microfilaments. Analysis of the peptides in these fractions, by sodium dodecyl sulfate polyacrylamide gel electrophoresis, indicated that although the actin peptide bands were prominent, a band apparently corresponding to myosin was drastically reduced. The morphological change was accompanied by a reduced bile flow and biliary excretion of bile acids, proteins and the exogenous organic anion dibromo-sulphthalein. These changes may result from a modification of the microfilaments, suggesting a role of the microfilaments in the liver membrane structure and function.

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