In vitro synthesis of IgG and IgM in patients with HBsAg-positive and HBsAg-negative chronic active hepatitis

Authors

  • Giovanni Triolo,

    1. Laboratory of Immunopathology, Veterans Administration Medical Center, Bronx, and the Department of Pathology and Medicine (Div. of Liver Diseases) Mount Sinai School of Medicine of the City University of New York, New York, U.S.A.
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  • Salvatore Nardiello,

    1. Laboratory of Immunopathology, Veterans Administration Medical Center, Bronx, and the Department of Pathology and Medicine (Div. of Liver Diseases) Mount Sinai School of Medicine of the City University of New York, New York, U.S.A.
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  • Evangelista Sagnelli,

    1. Laboratory of Immunopathology, Veterans Administration Medical Center, Bronx, and the Department of Pathology and Medicine (Div. of Liver Diseases) Mount Sinai School of Medicine of the City University of New York, New York, U.S.A.
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  • Fenton Schaffner,

    1. Laboratory of Immunopathology, Veterans Administration Medical Center, Bronx, and the Department of Pathology and Medicine (Div. of Liver Diseases) Mount Sinai School of Medicine of the City University of New York, New York, U.S.A.
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  • Salvatore Vernace,

    1. Laboratory of Immunopathology, Veterans Administration Medical Center, Bronx, and the Department of Pathology and Medicine (Div. of Liver Diseases) Mount Sinai School of Medicine of the City University of New York, New York, U.S.A.
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  • Fiorenzo Paronetto

    Corresponding author
    1. Laboratory of Immunopathology, Veterans Administration Medical Center, Bronx, and the Department of Pathology and Medicine (Div. of Liver Diseases) Mount Sinai School of Medicine of the City University of New York, New York, U.S.A.
      Laboratory Service VA Medical Center 130 West Kingsbridge Road Bronx, New York 10468 U.S.A.
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Laboratory Service VA Medical Center 130 West Kingsbridge Road Bronx, New York 10468 U.S.A.

Abstract

ABSTRACT— Spontaneous and PWM-driven IgG and IgM synthesis was investigated in the PBMC of 15 patients with HBsAg-negative CAH and six HBsAg-positive patients with CAH. PBMC from patients with HBsAg-positive CAH show an impaired IgG synthesis upon stimulation with PWM but an IgM increase similar to that of control subjects. In contrast, PBMC from HBsAg-negative patients with CAH show a trend to spontaneous increased synthesis of IgG and a decrease or lack of IgG and IgM synthesis upon PWM stimulation. Steroid treatment seems to ameliorate these alterations. No differences were found among the three groups of HBsAg-negative chronic active hepatitis (autoimmune, HBsAg-related and cryptogenic). These results indicate that differences of B-cell functions may exist in the two groups of CAH patients, not only in spontaneous B-cell activation or PWM-induced Ig synthesis but also in the different classes of Ig.

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