In situ hybridizaiton in Viral hepatitis

Authors

  • F. Nagro,

    Corresponding author
    1. Disions of Gastroentrerology, Molientte Hospital and Dapartment of Biomedical Sciences and Human Onclolgy, University of Turin, Turin, Italy
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  • D. Pacchiionl,

    1. Disions of Gastroentrerology, Molientte Hospital and Dapartment of Biomedical Sciences and Human Onclolgy, University of Turin, Turin, Italy
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  • A. Meadardini,

    1. Disions of Gastroentrerology, Molientte Hospital and Dapartment of Biomedical Sciences and Human Onclolgy, University of Turin, Turin, Italy
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  • G. Bussolati,

    1. Disions of Gastroentrerology, Molientte Hospital and Dapartment of Biomedical Sciences and Human Onclolgy, University of Turin, Turin, Italy
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  • F. Bonine

    1. Disions of Gastroentrerology, Molientte Hospital and Dapartment of Biomedical Sciences and Human Onclolgy, University of Turin, Turin, Italy
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Division of Gastroenterology, Mollnette Hospital, Corso Bramante 88, 10126 Turin, Italy, Fax #0039-11-677118

Abstract

Abstract: In situ hybridizaiton (ISH) is a sensitive and specific technique for detecting nucleic acids in intact cells. Visualization of the target sequences by autoradiography or immunohistochemistry allows their precise subcellular localization and quantitation. The application of ISH techniques has contributed to the understanding of the complex replicative cycle of hepatitis B virus. More recently, hepatitis delta and C virus replication has also been studied by this technique. ISH-based assays have finally been used to follow the replication of cytomegalovirus within the transplanted liver. Although ISH is a powerful tool for the molecular biologist, its clinical significance in the diagnosis and prognosis of human I hepatitis virus infections has yet to be fully evaluated.

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