The use of radioactively labelled riboprobes for in situ hybridizaiotn; background and examples of applicaiton


Laboratory of Neurobiology, Department of Animal Biology, University of Turin, via Accademia Albertina 17, 10123 Turin, Italy


Abstract: An overivew is provided concerning the preparation and use of radiolabelled single-stranded RNA probes (ribooprobes) for in situ hybridization. All theoretical aspects are covered as weel as some practical examples from the authors' experience. The use of riboprobes for in situ hybridizaiton represents a valid and highly sensitive alternative to oligonucleotide probes. A sampel protocol is finally described in detail: this should enable any investigators to design the ISH assay that best fits their particular needs.