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Keywords:

  • autoradiography;
  • in situ hybridization;
  • radiolabelled probes;
  • nuclear emulsion

Abstract: In the context of in situ hybridization techniques, autoradiography is used to localize radiolabelled probes within intact tissue sections or cell preparations. The present report describes in detail a standardized autoradiographic procedure. Topics include the choice of the emulsion, its preparation and layering onto the slides, the development procedure and the analysis of the results. Special attention is devoted to the practical aspects, so that the technique will be generally applicable, according to the reader's needs.