Non-readioactive nucleiec acid probes: labelling and detection procedures

Authors

  • Gluseppe Vale,

    Corresponding author
    1. Department of pathology, University of Milan School of Medicine, Milan, Italy
      Department of pathology, university of Milan School of Medicine, institute of Biomedical Sciences, Ospedale S. Paolo, Via A. Di Rudini 8, 201142 Milan, Italy
    Search for more papers by this author
  • Patrizia Dell'Orto

    1. Department of pathology, University of Milan School of Medicine, Milan, Italy
    Search for more papers by this author

Department of pathology, university of Milan School of Medicine, institute of Biomedical Sciences, Ospedale S. Paolo, Via A. Di Rudini 8, 201142 Milan, Italy

Abstract

Abstract: A comprehensive overview is given concerning the in situ hybridizaiton tecnhiques using non-radioactively labelled probes. Details are given on the preparation of the probes with different reporter moelecules and on the corresponding revealing assays. The development of non-isotopic labeeling methods for nucleic acid probes has facilitated the diffusion of the in situ hybridizaiton techniques to the research as well as to the clinical pathology laboratory. Non-radioactive probes have serveral advantages over the radiolabelled ones, including cost, shelf-life, safety and, last but not least. resolutions of the signal.

Ancillary