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Keywords:

  • non isotopic in situ hybridizaiton;
  • sulphonated probes;
  • human immunodeficiency virus-1;
  • acquired immune deficiency;
  • syndrome

Abstract: A detailed procedure is described that allows detection of the presence of human immunodeficiency virus-1 (HIV-1) transcripts within both acetone-fixed tisseues and peripheral blood mononuclear cells. This assay uses cDNA probes labelled by a non-procedure that results in the modification of cytosine residues trhough covalent linkage to a sulophone group. In sit hybridized probe is than detected by an alkaline phostphatase-conjugated antibody specifically directed againsts the sulphone hapten. This procedure is specific, rapid and safe and can be applied in the research as well as in the clinical pathology settings.