Oxidative stress in hepatocytes and stimulatory state of Kupffer cells after reperfusion differ between warm and cold ischemia in rats

Authors


Third Department of Internal Medicine, Saitama Medical School, 38 Morohongo, Moroyama-cho, Iruma-gun, Saitama 350–04, Japan

Abstract

Rat liver was kept at 4°C or 37°C in MEM, and reperfused through a closed circulation from the hepatic vein to the portal vein at 37°C with the same solution. Although purine nucleoside phosphorylase and ALT activities were increased in the perfusate, depending on the duration of ischemia at both 4°C and 37°C, the ratio of the latter to the former was significantly higher after 37°C-ischemia than after 4°C-ischemia. The stimulation stage of Kupffer cells evaluated in situ by formazan deposition after liver perfusion with nitro blue tetrazolium and phorbol myristate acetate was elevated after 4°C-ischemia longer than 1 h, but not after 37°C-ischemia. In contrast, the degree of oxidative stress in hepatocytes assessed by formazan deposition after liver perfusion with nitro blue tetrazolium alone was greater after 37°C-ischemia than after 4°C-ischemia. These results suggest that oxidative stress in hepatocytes and the stimulatory state of Kupffer cells after ischemia-reperfusion may differ between 4°C-ischemia and 37°C-ischemia, probably leading to different development of liver damage.

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