Effect of UV-B (302 nm) irradiation on isolated rat hepatocytes


Department of Gastroenterology, Osmania General Hospital, Afzalgunj, Hyderabad, Andhra Pradesh, Pin-500 012, India


Abstract: The present study aimed to evaluate the effect of UV-B irradiation on the functional integrity, and the metabolic and detoxifying capacity of isolated rat hepatocytes. Isolated rat hepatocytes were irradiated in various doses (400 Jm-2, 600 Jm-2, 800 Jm-2 and 1000 Jm-2). The cells were assayed for total lactate dehydrogenase, Na+-K+-ATPase, ornithine carbamyltransferase activity (OCT) and urea production capacity. Lactate dehydrogenase and Na+-K+-ATPase activity were significantly decreased in all four irradiated groups (P>0.001), whereas viability, OCT and urea production capacity showed no alterations.