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Keywords:

  • isolated hepatocytes;
  • lactate dehydrogenase;
  • Na+-K+-ATPase;
  • ornithine carbamyl transferase;
  • urea;
  • UV-B irradiation

Abstract: The present study aimed to evaluate the effect of UV-B irradiation on the functional integrity, and the metabolic and detoxifying capacity of isolated rat hepatocytes. Isolated rat hepatocytes were irradiated in various doses (400 Jm-2, 600 Jm-2, 800 Jm-2 and 1000 Jm-2). The cells were assayed for total lactate dehydrogenase, Na+-K+-ATPase, ornithine carbamyltransferase activity (OCT) and urea production capacity. Lactate dehydrogenase and Na+-K+-ATPase activity were significantly decreased in all four irradiated groups (P>0.001), whereas viability, OCT and urea production capacity showed no alterations.