Assessment of nutritional status in rhesus monkeys: comparison of dual-energy X-ray absorptiometry and stable isotope dilution

  1. Stéphane Blanc1,
  2. Ricki Colman2,
  3. Joseph Kemnitz2,3,
  4. Richard Weindruch4,5,
  5. Scott Baum2,
  6. Jon Ramsey5,
  7. Dale Schoeller1

Article first published online: 27 APR 2005

DOI: 10.1111/j.1600-0684.2005.00106.x

Issue

Journal of Medical Primatology

Journal of Medical Primatology

Volume 34, Issue 3, pages 130–138, June 2005

Additional Information

How to Cite

Blanc, S., Colman, R., Kemnitz, J., Weindruch, R., Baum, S., Ramsey, J. and Schoeller, D. (2005), Assessment of nutritional status in rhesus monkeys: comparison of dual-energy X-ray absorptiometry and stable isotope dilution. Journal of Medical Primatology, 34: 130–138. doi: 10.1111/j.1600-0684.2005.00106.x

Author Information

  1. 1

    Department of Nutritional Sciences, University of Wisconsin–Madison, Madison, WI

  2. 2

    Wisconsin National Primate Research Center, University of Wisconsin–Madison, Madison, WI

  3. 3

    Department of Physiology, University of Wisconsin–Madison, Madison, WI, and Veterans Administration Hospital, Geriatric Research, Education and Clinical Center, Madison, WI

  4. 4

    Department of Medicine, University of Wisconsin–Madison, Madison, WI

  5. 5

    Department of Molecular Biosciences, School of Veterinary Medicine, University of California–Davis, Davis, CA, USA

*Stéphane Blanc, Centre Ecologie et Physiologie Energétiques, CNRS UPR 9010, 23 rue Becquerel, 67087 Strasbourg Cedex 02, France. Tel.: +33 3 88 10 69 42; fax: +33 3 88 10 69 06; e-mail: stephane.blanc@c-strasbourg.fr

Publication History

  1. Issue published online: 27 APR 2005
  2. Article first published online: 27 APR 2005
  3. Accepted November 17, 2004.

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Keywords:

  • absorptiometry;
  • stable isotopes;
  • body composition

Abstract:  Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.

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