Anti-CeHV1 antibodies of two cynomolgus macaques cross-react with HSV2 but not HSV1 antigens in ELISA

  1. Edwin Coutrot1,
  2. Marie Blancher-Sardou2,
  3. Antoine Blancher1

Article first published online: 24 JUN 2007

DOI: 10.1111/j.1600-0684.2007.00233.x

Issue

Journal of Medical Primatology

Journal of Medical Primatology

Volume 37, Issue 1, pages 38–44, February 2008

Additional Information

How to Cite

Coutrot, E., Blancher-Sardou, M. and Blancher, A. (2008), Anti-CeHV1 antibodies of two cynomolgus macaques cross-react with HSV2 but not HSV1 antigens in ELISA. Journal of Medical Primatology, 37: 38–44. doi: 10.1111/j.1600-0684.2007.00233.x

Author Information

  1. 1

     Laboratoire d'Immunogénétique Moléculaire, Université Paul Sabatier, Toulouse, France

  2. 2

     Laboratoire d'Analyses Médicales, Toulouse, France

*Antoine Blancher, Laboratoire d'immunologie, Hôpital Rangueil, TSA50032, 31059 Toulouse cedex 9, France. Tel.: +33 561 32 3432; fax: +33 561 32 3424; e-mail: blancher.a@chu-toulouse.fr

Publication History

  1. Issue published online: 24 JUN 2007
  2. Article first published online: 24 JUN 2007
  3. Accepted May 21, 2007.

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Keywords:

  • B virus;
  • cross-reactivity;
  • serology

Abstract

Background  The aim of the study was to compare the cross-reactivity of macaque anti-CeHV1 antibodies with type 1 and type 2 human herpes simplex viruses (HSV1 and HSV2).

Methods  We studied the serum of 344 animals which had been tested either positive (n = 39) or negative (n = 305) for the presence of CeHV1 antibodies by expert laboratories. Macaque serums were studied by means of two ELISA: one based on HSV1 antigen-coated wells, the other on polystyrene beads coated with HSV1 and HSV2 antigens in approximately equal proportions.

Results  In the serum of two animals originating from Vietnam, we found anti-CeHV1 antibodies cross-reacting with HSV2 but not with HSV1 antigens. For the serum with the highest titer, inhibition by soluble antigens confirmed the high affinity of the antibodies for HSV2 antigens.

Conclusions  Tests using HSV1 and HSV2 in a combined way are better suited to macaque screening than tests using only HSV1 antigens.

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