Both authors contributed equally to this work.
Expression of β2-adrenergic receptor in oral squamous cell carcinoma
Article first published online: 30 DEC 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Munksgaard
Journal of Oral Pathology & Medicine
Volume 38, Issue 4, pages 371–376, April 2009
How to Cite
Shang, Z. J., Liu, K. and Liang, D. F. (2009), Expression of β2-adrenergic receptor in oral squamous cell carcinoma. Journal of Oral Pathology & Medicine, 38: 371–376. doi: 10.1111/j.1600-0714.2008.00691.x
- Issue published online: 20 MAR 2009
- Article first published online: 30 DEC 2008
- Accepted for publication May 6, 2008
- oral squamous cell carcinoma;
- tumor metastasis;
- β2-adrenergic receptor
Background: It has been speculated that chemokines and neurotransmitters might be involved in the organ-specific development of metastases because cancer metastasis is similar to the regulation of migratory activity in leukocytes. Here, we aimed to examine the expression of β2-adrenergic receptor (β2-AR) in oral squamous cell carcinoma (OSCC), and to investigate its correlation with tumor development and metastasis.
Methods: Expression of β2-AR was examined in 65 cases of OSCC specimens, 10 cases of normal oral mucosa, and two cell lines using immunohistochemistry, Western blot and RT-PCR. The differences in β2-AR expression between various groups were evaluated using SPSS 13.0 Statistical Software. Cell proliferation assays were assayed by β-adrenergic receptors agonists (norepinephrine) and antagonists (propranolol). Norepinephrine-mediated cell migration was assayed in Matrigel-coated chemotaxis chamber.
Results: β2-AR was highly expressed on OSCC compared to normal controls. In OSCC, positive β2-AR expression was significantly correlated with cervical lymph node metastasis (P = 0.001), age (P = 0.003), tumor size (P = 0.001) and clinical stage (P = 0.001), but not with gender. RT-PCR and Western blot also confirmed positive β2-AR expression in OSCC and TCa8113 cell line, and negative β2-AR expression in normal oral mucosa and ACC cell line. β-adrenoreceptor agonist (norepinephrine) was a potent mitogen for TCa8113 and ACC cell lines, and completely inhibited by the selective antagonist of β-adrenergic receptors (propranolol). Norepinephrine induced migratory activity of OSCC cells in a dose-dependent manner.
Conclusion: Increased expression of β2-AR may play an important role in the formation and metastasis of OSCC.