Developmental expression of solute carrier family 26A member 4 (SLC26A4/pendrin) during amelogenesis in developing rodent teeth

Authors

  • Antonius L. J. J. Bronckers,

    1. Department of Oral Cell Biology ACTA, University of Amsterdam and VU-University of Amsterdam, Research Institute MOVE, Amsterdam, the Netherlands
    Search for more papers by this author
  • Jing Guo,

    1. Department of Oral Cell Biology ACTA, University of Amsterdam and VU-University of Amsterdam, Research Institute MOVE, Amsterdam, the Netherlands
    Search for more papers by this author
  • Behrouz Zandieh-Doulabi,

    1. Department of Oral Cell Biology ACTA, University of Amsterdam and VU-University of Amsterdam, Research Institute MOVE, Amsterdam, the Netherlands
    Search for more papers by this author
  • Theodore J. Bervoets,

    1. Department of Oral Cell Biology ACTA, University of Amsterdam and VU-University of Amsterdam, Research Institute MOVE, Amsterdam, the Netherlands
    Search for more papers by this author
  • Donacian M. Lyaruu,

    1. Department of Oral Cell Biology ACTA, University of Amsterdam and VU-University of Amsterdam, Research Institute MOVE, Amsterdam, the Netherlands
    Search for more papers by this author
  • Xiangming Li,

    1. Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA
    Search for more papers by this author
  • Philine Wangemann,

    1. Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA
    Search for more papers by this author
  • Pamela DenBesten

    1. Department of Orofacial Sciences, University of California-San Francisco, San Francisco, CA, USA
    Search for more papers by this author

Antonius L. J. J. Bronckers, Department of Oral Cell Biology, ACTA, Vrije Universiteit, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, the Netherlands

Telefax: +31–020–5980333
E-mail: a.bronckers@acta.nl

Abstract

Bronckers ALJJ, Guo J, Zandieh-Doulabi B, Bervoets TJ, Lyaruu DM, Li X, Wangemann P, DenBesten P. Developmental expression of solute carrier family 26A member 4 (SLC26A4/pendrin) during amelogenesis in developing rodent teeth.
Eur J Oral Sci 2011; 119 (Suppl. 1): 185–192. © 2011 Eur J Oral Sci

Ameloblasts need to regulate pH during the formation of enamel crystals, a process that generates protons. Solute carrier family 26A member 4 (SLC26A4, or pendrin) is an anion exchanger for chloride, bicarbonate, iodine, and formate. It is expressed in apical membranes of ion-transporting epithelia in kidney, inner ear, and thyroid where it regulates luminal pH and fluid transport. We hypothesized that maturation ameloblasts express SLC26A4 to neutralize acidification of enamel fluid in forming enamel. In rodents, secretory and maturation ameloblasts were immunopositive for SLC26A4. Staining was particularly strong in apical membranes of maturation ameloblasts facing forming enamel. RT-PCR confirmed the presence of mRNA transcripts for Slc26a4 in enamel organs. SLC26A4 immunostaining was also found in mineralizing connective tissues, including odontoblasts, osteoblasts, osteocytes, osteoclasts, bone lining cells, cellular cementoblasts, and cementocytes. However, Slc26a4-null mutant mice had no overt dental phenotype. The presence of SLC26A4 in apical plasma membranes of maturation ameloblasts is consistent with a potential function as a pH regulator. SLC26A4 does not appear to be critical for ameloblast function and is probably compensated by other pH regulators.

Ancillary