Expression Studies of Pigmentation and POU-Domain Genes in Human Melanoma Cells

Authors

  • RICHARD A. STURM,

    Corresponding author
    1. Centre for Molecular Biology and Biotechnology, The University of Queensland, Brisbane, Qld 4072;
      Address reprint requests to Dr. Richard A. Sturm, Centre for Molecular Biology and Biotechnology, University of Queensland, Qld 4072, Australia.
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  • BRENDAN J. O'SULLIVAN,

    1. Centre for Molecular Biology and Biotechnology, The University of Queensland, Brisbane, Qld 4072;
    2. Queensland Cancer Fund Research Unit, Queensland Institute for Medical Research Herston, Qld 4029, Australia
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  • J. ANGUS F. THOMSON,

    1. Centre for Molecular Biology and Biotechnology, The University of Queensland, Brisbane, Qld 4072;
    2. Queensland Cancer Fund Research Unit, Queensland Institute for Medical Research Herston, Qld 4029, Australia
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  • NEGAR JAMSHIDI,

    1. Centre for Molecular Biology and Biotechnology, The University of Queensland, Brisbane, Qld 4072;
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  • JULIE PEDLEY,

    1. Queensland Cancer Fund Research Unit, Queensland Institute for Medical Research Herston, Qld 4029, Australia
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  • PETER G. PARSONS

    1. Queensland Cancer Fund Research Unit, Queensland Institute for Medical Research Herston, Qld 4029, Australia
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Address reprint requests to Dr. Richard A. Sturm, Centre for Molecular Biology and Biotechnology, University of Queensland, Qld 4072, Australia.

Abstract

Human melanoma cell lines have been used to examine the regulation of the tyrosinase (TYR) and tyrosinase-related protein genes TRP-1 and TRP-2 in response to differentiating chemicals and UV irradiation. TRP-1 mRNA levels can be repressed by treatment with the differentiating chemicals DMSO and HMBA. There is little effect of UV irradiation on pigment synthesis by human melanoma cell lines or tyrosinase activity, with variable effects on the levels of the TYR, TRP-1, and TRP-2 gene transcripts. The human TRP-1 gene promoter has been isolated and its activity tested by transient cell transfection to begin an examination of signal transduction mechanisms operating in response to pigmenting and differentiating agents. To identify transcription factors that may be involved in melanocytic gene expression, we studied the N-Oct-3 and N-Oct-5 octamer-binding activities normally expressed in the neuroectodermal cell lineage and which are expressed at high levels in melanoma cells. POU-domain-containing cDNA have been isolated from the A2058 human melanoma cell line that are homologous to the brn-2 gene that encodes N-Oct-3 and N-Oct-5.

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