The Administration of an α-MSH Analogue Reduces the Serum Release of IL-1α and TNFα Induced by the Injection of a Sublethal Dose of Lipopolysaccharides in the BALB/c Mouse

Authors

  • CHRISTOPHE GONINDARD,

    1. Institut Européen de Biologie Cellulaire et de Cytopathologie, Parc technologique du canal, Villa Missouri, 18, Avenue de l'Europe, 31520 Ramonville-Saint-Agne, France;
    2. Laboratoire de Biologie Cellulaire, Université Paul Sabatier, 38, rue des 36 ponts, 31400 Toulouse, France
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  • CATHERINE GOIGOUX,

    1. Institut Européen de Biologie Cellulaire et de Cytopathologie, Parc technologique du canal, Villa Missouri, 18, Avenue de l'Europe, 31520 Ramonville-Saint-Agne, France;
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  • ETIENNE HOLLANDE,

    1. Laboratoire de Biologie Cellulaire, Université Paul Sabatier, 38, rue des 36 ponts, 31400 Toulouse, France
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  • LUCIEN DUSSOURD D'HINTERLAND

    Corresponding author
    1. Institut Européen de Biologie Cellulaire et de Cytopathologie, Parc technologique du canal, Villa Missouri, 18, Avenue de l'Europe, 31520 Ramonville-Saint-Agne, France;
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Address reprint requests to Lucien Dussourd D'Hinterland, Institut Européen de Biologie Cellulaire et de Cytopathologie, Parc technologique du canal, Villa Missouri, 18, Avenue de l'Europe, 31520. Ramonville-Saint-Agne, France.

Abstract

The injection of α-MSH or of one of its analogues ([Nle4-D.Phe7] α-MSH4–10) reduced, in vivo, the release of two cytokines (IL-1α and TNFα) involved in inflammation. The inflammatory state was induced in BALB/c mice by intraperitoneal injection of a sublethal dose of lipopolysaccharides (LPS). The assay of these cytokines by ELISA showed a reduction of 20% with α-MSH and between 30 and 60% with the α-MSH analogue. The α-MSH or the analogue was administered in one of two ways: intravenously or subcutaneously. The most efficient method seemed to be the subcutaneous one because it improved the activity 10,000 times more than the intravenous method. Moreover, the analogue induced a regression of mortality in the animals treated by the intravenous method. Our results show that α-MSH and one of its analogues inhibit IL-1α and TNFα, and can be used as anti-inflammatory molecules.

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