Transcription of Melanogenesis Enzymes in Melanocytes: Dependence upon Culture Conditions and Co-Cultivation With Keratinocytes
Article first published online: 28 JUL 2006
Pigment Cell Research
Volume 9, Issue 4, pages 179–184, August 1996
How to Cite
KIPPENBERGER, S., BERND, A., BEREITER-HAHN, J., RAMIREZ-BOSCA, A., KAUFMANN, R. and HOLZMANN, H. (1996), Transcription of Melanogenesis Enzymes in Melanocytes: Dependence upon Culture Conditions and Co-Cultivation With Keratinocytes. Pigment Cell Research, 9: 179–184. doi: 10.1111/j.1600-0749.1996.tb00107.x
- Issue published online: 28 JUL 2006
- Article first published online: 28 JUL 2006
- Received May 16, 1996; accepted July 10, 1996.
- TRP 1;
- TRP 2
Eumelanogenesis of human skin melanocytes requires at least three enzymes: tyrosinase, TRP 1, and TRP 2. The regulation of these enzymes on transcriptional level was detected in a semiquantitative attempt. The total RNA of melanocytes was reverse-transcripted and followed by a PCR with degenerated primers for all three enzymes. The amplification products were related to each other densitometrically. We examined five different culture conditions: 1) melanocytes in a popular phorbolester containing F-10-medium, 2) melanocytes in a co-culture medium with EGF, 3) melanocytes in a co-culture medium with high calcium, 4) melanocytes co-cultured with keratinocytes in EGF containing co-culture medium, and 5) melanocytes co-cultured with keratinocytes in co-culture medium with high calcium. Melanocytes cultured in phorbolester containing F-10-medium featured transcripts of tyrosinase, TRP 1, and TRP 2 in the ratio 45:45:10. The same results were obtained for melanocytes co-cultured with keratinocytes under the two different culture conditions. In melanocytes cultured alone in co-culture media only TRP 1-transcripts were present. It is likely that under co-culture conditions a keratinocyte-derived factor supports the transcription of all three enzymes. For melanocytes in the phorbolester-containing melanocyte medium a proteinkinase C dependent regulation of transcription seems possible.