The authors contributed equally to this work.
Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds
Article first published online: 16 MAR 2007
Pigment Cell Research
Volume 20, Issue 2, pages 120–127, April 2007
How to Cite
Choi, T.-Y., Kim, J.-H., Ko, D. H., Kim, C.-H., Hwang, J.-S., Ahn, S., Kim, S. Y., Kim, C.-D., Lee, J.-H. and Yoon, T.-J. (2007), Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds. Pigment Cell Research, 20: 120–127. doi: 10.1111/j.1600-0749.2007.00365.x
- Issue published online: 16 MAR 2007
- Article first published online: 16 MAR 2007
- Received 17 October 2006, revised and accepted for publication 29 December 2006
- melanogenic regulatory compounds;
- phenotype-base screening;
Although many hypo-pigmenting agents are currently available, the demand for novel whitening agents is increasing, in part due to the weak effectiveness and unwanted side effects of currently available compounds. To screen for novel hypo-pigmenting agents, many methodologies such as cell culture and enzymatic assays are routinely used. However, these models have disadvantages in terms of physiological and economic relevance. In this study, we validated zebrafish as a whole-animal model for phenotype-based screening of melanogenic inhibitors or stimulators. We used both the well-known melanogenic inhibitors (1-phenyl-2-thiourea, arbutin, kojic acid, 2-mercaptobenzothiazole) and newly developed small molecule compounds (haginin, YT16i). All the tested compounds produced inhibitory effects on the pigmentation of zebrafish, most likely due to their inhibitory potential on tyrosinase activity. In simultaneous in vivo toxicity tests, a newly developed melanogenic inhibitor YT16i showed massive abnormalities in terms of deformed morphologies and cardiac function. Together, these results provide a rationale in screening and evaluating the putative melanogenic regulatory compounds. We suggest that the zebrafish system is a novel alternative to mammalian models, with several advantages including the rapidity, cost-effectiveness, and physiological relevance.