Human periodontal ligament: a niche of neural crest stem cells
Article first published online: 4 JUL 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Munksgaard
Journal of Periodontal Research
Volume 43, Issue 5, pages 531–536, October 2008
How to Cite
Coura, G. S., Garcez, R. C., De Aguiar, C. B. N. M., Alvarez-Silva, M., Magini, R. S. and Trentin, A. G. (2008), Human periodontal ligament: a niche of neural crest stem cells. Journal of Periodontal Research, 43: 531–536. doi: 10.1111/j.1600-0765.2007.01065.x
- Issue published online: 26 AUG 2008
- Article first published online: 4 JUL 2008
- Accepted for publication November 1, 2007
- neural crest;
- periodontal ligament;
- stem cell;
- cell culture
Background and Objective: The periodontal ligament is a specialized connective tissue, derived from dental follicle and originated from neural crest cells. Recently it has been suggested, based on animal models, that periodontal ligament could be a niche for neural crest stem cells. However, there is still little knowledge on this subject. The identification of neural crest adult stem cells has received much attention based on its potential in tissue regeneration. The objective of the present work was to verify the human periodontal ligament as a niche for neural crest stem cells.
Material and Methods: Cells from human periodontal ligament were isolated from 10 teeth of seven individuals (periodontal ligament pool group) and also from four teeth of one individual (periodontal ligament single group), after enzymatic digestion. The cells were cultured in specific inductive medium. Analyses of protein and gene expression were performed through immunocytochemistry and reverse transcription–polymerase chain reaction techniques, respectively.
Results: Mesodermal phenotypes (adipogeneic, osteogenic and myofibroblastic) were identified after culture in inductive medium. Immunocytochemistry analyses showed the presence of the nestin marker of neural stem cells and also markers of undifferentiated neural crest cells (HNK1, p75). When cultured in inductive medium that allowed neural differentiation, the cells showed markers for β-tubulin III, neurofilament M, peripherin, microtubule-associated protein 2 and protein zero. The results were similar between the two study groups (the periodontal ligament pool group and the periodontal ligament single group).
Conclusion: This research provides evidence that human periodontal ligament, in addition to its mesodermal derivatives, produces neural crest-like cells. Such features suggest a recapitulation of their embryonic state. The human periodontal ligament revealed itself as a viable alternative source for possible primitive precursors to be used in stem-cell therapies.