Expression of matrix metalloproteinase-1, matrix metalloproteinase-2 and extracellular metalloproteinase inducer in human periodontal ligament cells stimulated with interleukin-1beta
Article first published online: 8 JUL 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Munksgaard
Journal of Periodontal Research
Volume 44, Issue 6, pages 784–793, December 2009
How to Cite
Xiang, J., Li, C., Dong, W., Cao, Z. and Liu, L. (2009), Expression of matrix metalloproteinase-1, matrix metalloproteinase-2 and extracellular metalloproteinase inducer in human periodontal ligament cells stimulated with interleukin-1beta. Journal of Periodontal Research, 44: 784–793. doi: 10.1111/j.1600-0765.2008.01191.x
- Issue published online: 23 OCT 2009
- Article first published online: 8 JUL 2009
- Accepted for publication November 23, 2008
- extracellular matrix metalloproteinase inducer;
- matrix metalloproteinases;
- periodontal ligament cells
Background and Objectives: Matrix metalloproteinases (MMPs), produced by both infiltrating and resident cells of the periodontium, play important roles in physiologic and pathologic events. Both interleukin-1beta and extracellular MMP inducer can stimulate the expression of MMPs, which in turn leads to breakdown of the periodontium. However, it is currently unknown whether interleukin-1beta up-regulates MMPs through stimulating the expression of extracellular MMP inducer. The aims of this study were to investigate the effect of interleukin-1beta on the expression of MMP-1, MMP-2 and extracellular MMP inducer in human periodontal ligament cells and to evaluate whether the regulation of MMP-1 and MMP-2 by this cytokine occurred through an effect on extracellular MMP inducer expression.
Material and Methods: Cultured human periodontal ligament cells were treated with varying concentrations (0.01–10 ng/mL) of interleukin-1beta at for 6, 12 and 24 h. Reverse transcription–polymerase chain reaction, enzyme-linked immunosorbent assay, gelatin zymography and western blotting were performed to measure the mRNA and protein levels of MMP-1, MMP-2 and extracellular MMP inducer.
Results: Basal levels of mRNA and protein for MMP-1, MMP-2 and extracellular MMP inducer were detected in untreated human periodontal ligament cells. Interleukin-1beta significantly up-regulated the expression of MMP-1 and MMP-2 mRNA and protein (p < 0.05); however, the levels of mRNA and protein for extracellular MMP inducer were not significantly different (p > 0.05). In the culture medium, the concentration of MMP-1 was also increased significantly, but the concentration of MMP-1 was not related to the concentration of extracellular MMP inducer (R2 = 0.2538, p > 0.05).
Conclusion: Interleukin-1beta up-regulated the levels of MMP-1 and MMP-2, but it did not alter the expression of extracellular MMP inducer. Expression of MMP-1 and MMP-2 might be elevated by interleukin-1beta and extracellular MMP inducer via two different signal pathways.