Periodontal tissue engineering with stem cells from the periodontal ligament of human retained deciduous teeth

Authors

  • K. Ji,

    1. Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, China
    2. Research and Development Center for Tissue Engineering, The Fourth Military Medical University, Xi’an, China
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    • These authors contributed equally to this work.

  • Y. Liu,

    1. Research and Development Center for Tissue Engineering, The Fourth Military Medical University, Xi’an, China
    2. Dental institute, General Hospital of Chinese PLA, Beijing, China
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    • These authors contributed equally to this work.

  • W. Lu,

    1. Department of Dentistry, The 461 Hospital of PLA, Changchun, China
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    • These authors contributed equally to this work.

  • F. Yang,

    1. Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, China
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  • J. Yu,

    1. Institute of Stomatology, Nanjing Medical University, Nanjing, China
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  • X. Wang,

    1. Research and Development Center for Tissue Engineering, The Fourth Military Medical University, Xi’an, China
    2. Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, China
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  • Q. Ma,

    1. Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, China
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  • Z. Yang,

    1. Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, China
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  • L. Wen,

    1. Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, China
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  • K. Xuan

    1. Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, China
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Kun Xuan, PhD, 145 West Changle Road, Xi’an, Shaanxi 710032, China
Tel: +86 29 84776087
Fax: +86 29 84776083
e-mail: xuankun@fmmu.edu.cn

Abstract

Background and Objective:  Periodontal ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in periodontal tissue engineering and regeneration. However, little knowledge is available on the periodontal ligament stem cells from human retained deciduous teeth (DePDLSCs). This study evaluated the potential of DePDLSCs in periodontal tissue regeneration.

Material and Methods:  DePDLSCs were isolated and purified by limited dilution. The characteristics of DePDLSCs were evaluated and compared with PePDLSCs both in vitro and in vivo.

Results:  DePDLSCs presented a higher proliferation rate and colony-forming capacity than PePDLSCs in vitro. During the osteogenic induction, alkaline phosphatase (ALP) activity, mineralized matrix formation and expression of mineralization-related genes, including runt-related transcription factor 2 (RUNX2), ALP, collagen type I (COLI) and osteocalcin (OCN) were significantly enhanced in DePDLSCs compared with PePDLSCs. Furthermore, DePDLSC cell sheets showed a stronger synthesis of collagen type I in the extracellular matrix than did PePDLSC cell sheets. After in vivo transplantation, DePDLSC cell sheets recombined with human dentin blocks were able to generate new cementum/periodontal ligament-like tissues.

Conclusion:  Our findings suggest that DePDLSCs can be used as a promising candidate for periodontal tissue engineering.

Ancillary