The antimicrobial effect of narrow-band UVB (313 nm) and UVA1 (345–440 nm) radiation in vitro

Authors


Städt. Hautklinik, Moltkestr. 120, D-71133 Karlsruhe, Germany

Abstract

Two new UV lamps were investigated with respect to their antibacterial effectiveness in vitro. Propioni (n=20 strains) and Micrococcaceae (n=16 strains) bacteria extracted from acne patients were applied to RCM and sheep blood agar plates and irradiated with a narrow-band UVB lamp (TL 100W/10R) at a wavelength of 313 nm and a UVA1 lamp (TL 01) at a wavelength of 345–440 nm. The precisely defined energy levels were, in the case of narrow-band UVB, 0.00, 0.30, 0.50, 1.00, 2.00 and 3.00 J/cm2 and, in the case of UVA1, 0.00, 2.50, 5.00, 7.50, 10.00 and 20.00 J/cm2. UVA1 inhibited neither the growth of Propioni nor Micrococcaceae bacteria. In contrast, the growth of Micrococcaceae was inhibited already at a dosage of 0.30 J/cm2 of narrow-band UVB (P<0.05), highly significant from 0.50 J/cm2 (P<0.01) and to a maximum of 2.2 powers of 10 at 3.00 J/cm2 compared with non-radiated control plates. Propioni bacteria were significantly inhibited at the minimum dosage of 0.30 J/cm2 of narrow-band UVB (P<0.01) and to a maximum of 2.8 powers of 10 at 3.00 J/cm2.

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