Iron chelation can modulate UVA-induced lipid peroxidation and ferritin expression in human reconstructed epidermis
Article first published online: 23 JAN 2004
Photodermatology, Photoimmunology & Photomedicine
Volume 20, Issue 1, pages 47–52, February 2004
How to Cite
Seité, S., Popovic, E., Verdier, M. P., Roguet, R., Portes, P., Cohen, C., Fourtanier, A. and Galey, J. B. (2004), Iron chelation can modulate UVA-induced lipid peroxidation and ferritin expression in human reconstructed epidermis. Photodermatology, Photoimmunology & Photomedicine, 20: 47–52. doi: 10.1111/j.1600-0781.2004.00064.x
- Issue published online: 23 JAN 2004
- Article first published online: 23 JAN 2004
- Accepted for publication 31 July 2003
- iron chelator;
- lipid peroxidation;
- reconstructed human epidermis;
Background/Purpose: As ferritin has been identified as an important factor in antioxidant defense in cultured human skin cells, we evaluated UVA-induced lipid hydroperoxides (LPO) production and ferritin expression in reconstructed human epidermis in vitro.
Results: Ferritin is regularly present in the basal layer of unirradiated epidermis both in the human skin in vivo and in the reconstructed human epidermis in vitro. Following acute UVA exposure, ferritin expression increased in basal epidermal cells in both models. Quantitative analysis showed that, in reconstructed human epidermis, LPO and ferritin levels increased linearly with the UVA dose. An iron chelator, OR10141, inhibited these inductions.
Conclusion: These findings demonstrate that reconstructed human epidermis is a useful in vitro model to study UVA-induced oxidative stress and protection afforded by iron chelators, antioxidants or UVA absorbers.