*These authors contributed equally.
Fox-P3-positive regulatory T cells are present in the skin of generalized atopic eczema patients and are not particularly affected by medium-dose UVA1 therapy
Article first published online: 22 MAY 2007
Photodermatology, Photoimmunology & Photomedicine
Volume 23, Issue 2-3, pages 81–85, April/June 2007
How to Cite
Schnopp, C., Rad, R., Weidinger, A., Weidinger, S., Ring, J., Eberlein, B., Ollert, M. and Mempel, M. (2007), Fox-P3-positive regulatory T cells are present in the skin of generalized atopic eczema patients and are not particularly affected by medium-dose UVA1 therapy. Photodermatology, Photoimmunology & Photomedicine, 23: 81–85. doi: 10.1111/j.1600-0781.2007.00284.x
- Issue published online: 22 MAY 2007
- Article first published online: 22 MAY 2007
- Accepted for publication 8 January 2007
Background: Regulatory T cells (T-reg cells) have been described as an important cell population in the UV treatment of inflammatory skin diseases.
Methods: We have treated five patients with generalized atopic eczema (AE) using medium-dose (15 cycles of 50 J/cm2, total dose of 750 J/cm2) UVA1 therapy and have analyzed the skin-infiltrating T-cellular subsets before and after therapy. Skin biopsies were split for immunohistochemistry and Real-time PCR and analyzed for CD4, Fox-P3, GATA-3, and IL-10 transcription as well as for CD3, CD4, CD152, Fox-P3, and GITR staining.
Results: In all the investigated patients, we observed a good clinical response to UVA1. As described previously, the number of epidermal T cells slightly declined after irradiation. However, we did not observe a general decrease in T cell numbers. Within the population of T cells, no specific difference in the kinetics of Fox-P3-positive cells and Fox-P3-specific mRNA was noted as compared with GATA-3 positive T cells. These results were paralleled by RT-PCR for IL-10 and staining for CD152, a surface molecule that has been described for T-reg cells.
Conclusion: In our hands, the clinical benefit of UVA1 treatment in AE patients does not seem to be due to a preferential survival/proliferation of T-reg cells.