Stability of melatonin in aqueous solution
Article first published online: 30 JAN 2007
Journal of Pineal Research
Volume 18, Issue 2, pages 90–92, March 1995
How to Cite
Cavallo, A. and Hassan, M. (1995), Stability of melatonin in aqueous solution. Journal of Pineal Research, 18: 90–92. doi: 10.1111/j.1600-079X.1995.tb00145.x
- Issue published online: 30 JAN 2007
- Article first published online: 30 JAN 2007
- Received November 14,1994; accepted Decem-ber 29,1994.
- melatonin stability
Abstract: Melatonin solutions are frequently used in human, animal, and in vitro research. Generally, fresh solutions are prepared, for fear of instability of mela-tonin in solution. We tested the high-performance liquid chromatography (HPLC) stability of melatonin in aqueous solutions stored room temperature, 4°C, and -70°C for up to 6 months. Solutions were prepared in a laminar flow hood using sterile technique, directly into sterile, pyrogen-free glass vacuum vials for storage. Different concentrations were tested (1.0–113.0 μg/ml). There was no loss of po-tency as assessed by HPLC, and the preparations remained sterile and pyrogen-free. We conclude that melatonin solutions may be prepared in batches maintained in sterile, pyrogen-free vials at 4°C or at -70°C until use within 6 months. This method will save on research time used for preparation of fresh solutions and will reduce the number of dose validation tests for each new experiment.