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Ventricular cerebrospinal fluid melatonin concentrations investigated with an endoscopic technique

Authors

  • Pierluigi Longatti,

    1. 1 Neurosurgical Unit –‘Ca'Foncello’ Hospital, University of Padova, Treviso ; 2 Department of Biochemistry, Clinical-Chemical Analysis Section, IRRCS Policlinico San Matteo, University of Pavia, Pavia ; 3 Department of Pharmaceutical Sciences, Faculty of Pharmacy and 4Department of Pharmacology and Anaesthesiology, University of Padova, Padova, Italy
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  • 1 Alessandro Perin,

    1. 1 Neurosurgical Unit –‘Ca'Foncello’ Hospital, University of Padova, Treviso ; 2 Department of Biochemistry, Clinical-Chemical Analysis Section, IRRCS Policlinico San Matteo, University of Pavia, Pavia ; 3 Department of Pharmaceutical Sciences, Faculty of Pharmacy and 4Department of Pharmacology and Anaesthesiology, University of Padova, Padova, Italy
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  • 1 Vittoria Rizzo,

    1. 1 Neurosurgical Unit –‘Ca'Foncello’ Hospital, University of Padova, Treviso ; 2 Department of Biochemistry, Clinical-Chemical Analysis Section, IRRCS Policlinico San Matteo, University of Pavia, Pavia ; 3 Department of Pharmaceutical Sciences, Faculty of Pharmacy and 4Department of Pharmacology and Anaesthesiology, University of Padova, Padova, Italy
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  • 2 Stefano Comai,

    1. 1 Neurosurgical Unit –‘Ca'Foncello’ Hospital, University of Padova, Treviso ; 2 Department of Biochemistry, Clinical-Chemical Analysis Section, IRRCS Policlinico San Matteo, University of Pavia, Pavia ; 3 Department of Pharmaceutical Sciences, Faculty of Pharmacy and 4Department of Pharmacology and Anaesthesiology, University of Padova, Padova, Italy
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  • 3 Pietro Giusti,

    1. 1 Neurosurgical Unit –‘Ca'Foncello’ Hospital, University of Padova, Treviso ; 2 Department of Biochemistry, Clinical-Chemical Analysis Section, IRRCS Policlinico San Matteo, University of Pavia, Pavia ; 3 Department of Pharmaceutical Sciences, Faculty of Pharmacy and 4Department of Pharmacology and Anaesthesiology, University of Padova, Padova, Italy
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  • and 4 Carlo Virgilio Luigi Costa 3

    1. 1 Neurosurgical Unit –‘Ca'Foncello’ Hospital, University of Padova, Treviso ; 2 Department of Biochemistry, Clinical-Chemical Analysis Section, IRRCS Policlinico San Matteo, University of Pavia, Pavia ; 3 Department of Pharmaceutical Sciences, Faculty of Pharmacy and 4Department of Pharmacology and Anaesthesiology, University of Padova, Padova, Italy
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Address reprint requests to Pietro Giusti, Department of Pharmacology and Anaesthesiology, University of Padova, Largo Meneghetti, 2, 35131 Padova, Italy.
E-mail: pietro.giusti@unipd.it

Abstract

Abstract:  The role of melatonin in humans still remains unclear. Uncertainties persist about its effects on neurophysiology regarding its levels in human cerebrospinal fluid (CSF), as the bulk of knowledge on this subject mainly derives from studies conducted on animals. In this study, CSF was micro-sampled with a simple, new method from each cerebral ventricle of patients undergoing neuroendoscopy for hydrocephalus. Our purpose was to measure CSF melatonin levels and determine possible differences in its concentration among various significant areas in the cerebral ventricles (e.g. pineal recess, pituitary recess, lateral ventricle, fourth ventricle) and lumbar cistern. From 2002 to 2004, 10 hydrocephalic patients were operated on using a neuroendoscopic technique. The CSF specimens were investigated for melatonin concentrations (free plus protein-bound) after deproteinization; the measurement technique was high-performance liquid chromatography. The preliminary data obtained with this endoscopic micro-sampling technique (applied to humans for the first time) suggest that melatonin is more concentrated within the ventricles and its highest concentration is found in the third ventricle (IIIv), although the difference detected between the CSF of the IIIv and that of the pineal recess was not significant.

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