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Movie S1. Ionomycin-induced cCa2+ stress and mCa2+ stress in RBA-1. (2E*) Time-lapse images of fluo-4. (2F*) Time-lapse images of rhod-2.

Movie S2. Ionomycin-induced ROS generation and △&PSgr;m depolarization in RBA-1. (4B*) Time-lapse images of ROS generation detected by DCF and dead nuclei by PI. (4C*) △&PSgr;m depolarization detected by TMRM. (4D*) Merged images of DCF, TMRM and PI.

Movie S3. Effect of the MPT-inhibition by CsA on ionomycin-induced mROS formation and △&PSgr;m depolarization in RBA-1. (6B*) ROS generation detected by DCF. (6C*) △&PSgr;m depolarization detected by TMRM. (6D*) Merged images of DCF and TMRM.

Movie S4. Effect of vitamin E on ionomycin-induced ROS generation and △&PSgr;m depolarization in RBA-1. (8B*) ROS generation detected by DCF. (8C*) △&PSgr;m depolarization detected by TMRM. (8D*) Merged images of DCF and TMRM.

Movie S5. Effect of melatonin on ionomycin-induced ROS formation and △&PSgr;m depolarization in RBA-1. (9B*) ROS generation detected by DCF. (9C*) △&PSgr;m depolarization detected by TMRM. (9D*) Merged images of DCF and TMRM.

Movie S6. Effect of melatonin on ‘oxidation free-Ca2+ stress’-mediated MPT in RBA-1. (10B*) ROS generation detected by DCF. (10C*) △&PSgr;m depolarization detected by TMRM. (10D*) Merged images of DCF and TMRM.

Movie S7. Effects of CsA plus vitamin E on ionomycin-induced ROS generation and △&PSgr;m depolarization in RBA-1. (13B*) ROS generation detected by DCF. (13C*) △&PSgr;m depolarization detected by TMRM. (13D*) Merged images of DCF and TMRM.

Movie S8. Effect of CsA and melatonin on ionomycin-induced ROS generation and △&PSgr;m depolarization in RBA-1. (14B*) ROS generation detected by DCF. (14C*) △&PSgr;m depolarization detected by TMRM. (14D*) Merged images of DCF and TMRM.

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