Melatonin protects against isoproterenol-induced myocardial injury in the rat: antioxidative mechanisms
Version of Record online: 4 MAR 2010
© 2010 The Authors. Journal compilation © 2010 John Wiley & Sons A/S
Journal of Pineal Research
Volume 48, Issue 3, pages 251–262, April 2010
How to Cite
Mukherjee, D., Roy, S. G., Bandyopadhyay, A., Chattopadhyay, A., Basu, A., Mitra, E., Ghosh, A. Kr., Reiter, R. J. and Bandyopadhyay, D. (2010), Melatonin protects against isoproterenol-induced myocardial injury in the rat: antioxidative mechanisms. Journal of Pineal Research, 48: 251–262. doi: 10.1111/j.1600-079X.2010.00749.x
- Issue online: 8 MAR 2010
- Version of Record online: 4 MAR 2010
- Received October 11, 2009; accepted December 23, 2009.
- hydroxyl radical;
- myocardial injury
Abstract: The present study was undertaken to explore the protective effect of melatonin against isoproterenol bitartrate (ISO)-induced myocardial injury in rat. Treatment of rats with ISO increased the level of lipid peroxidation products and decreased the reduced glutathione levels in cardiac tissue indicating that this synthetic catecholamine induces oxidative damage following oxidative stress. Pretreatment of ISO-injected rats with melatonin at a dose of 10 mg/kg body weight, i.p. prevented these changes. Additionally, melatonin also restored the activities and the levels of antioxidant enzymes which were found to be altered by ISO treatment. Treatment of rats with ISO resulted into an increased generation of hydroxyl radicals with melatonin pretreatment significantly reducing their production. Finally, treatment of rats with ISO caused a lowering of systolic pressure with reduced cardiac output and diastolic dysfunction whereas melatonin pretreatment significantly restored many of these parameters to normal. The findings document melatonin’s ability to provide cardio protection at a low pharmacological dose. Melatonin has virtually no toxicity which raises the possibility of this indole being a therapeutic treatment for ischemic heart disease.