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Figure S1. A representative image showing autophagy activation by MDC staining with or without OGD treatment in bEnd.3 cells. Scale bar = 25 μm.

Figure S2. The co-localization of cathepsin B (red fluorescence) with vWF (green fluorescence) in microvessels of ME rats is shown in a series of XZ reconstructions. DAPI counterstaining indicates cell nuclei (blue). Scale bar = 100 μm.

Figure S3. Proteins were separated by SDS-PAGE and were analyzed by Western blotting using anti-Beclin 1, -LC3, -Lamp2 and -cathepsin B antibodies. A representative image showing temporal expression of the indicated proteins following ME. β-actin antibody was used to equalize the immunodetection signals for protein loading.

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