The presence of melatonin in grapevine (Vitis vinifera L.) berry tissues

Authors

  • Sara Vitalini,

    1. Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milano, Italy
    2. Orto Botanico ‘G. E. Ghirardi’, Dipartimento di Biologia, Università degli Studi di Milano, Toscolano Maderno, Brescia, Italy
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  • Claudio Gardana,

    1. DiSTAM – Sezione Nutrizione, Università degli Studi di Milano, Milano, Italy
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  • Alessandro Zanzotto,

    1. CRA, Centro di Ricerca per la Viticoltura, Conegliano Veneto, Treviso, Italy
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  • Paolo Simonetti,

    1. DiSTAM – Sezione Nutrizione, Università degli Studi di Milano, Milano, Italy
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  • Franco Faoro,

    1. Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milano, Italy
    2. CNR, Dipartimento Agroalimentare, Istituto di Virologia Vegetale, Sezione di Milano, Milano, Italy
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  • Gelsomina Fico,

    1. Orto Botanico ‘G. E. Ghirardi’, Dipartimento di Biologia, Università degli Studi di Milano, Toscolano Maderno, Brescia, Italy
    2. Dipartimento di Biologia, Università degli Studi di Milano, Milano, Italy
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  • Marcello Iriti

    1. Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milano, Italy
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Address reprint requests to Marcello Iriti, Dipartimento di Produzione Vegetale, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy.
E-mail: marcello.iriti@unimi.it

Abstract

Abstract:  Melatonin has been reported in a variety of food plants and, consequently, in a number of plant-derived foodstuffs. In grapevine (Vitis vinifera L.) products, it was found in berry exocarp (skin) of different cultivars and monovarietal wines. Herein, we assessed, by means of mass spectrometry, the occurrence of melatonin in all berry tissues (skin, flesh, and seed) at two different phenological stages, pre-véraison and véraison. We detected the highest melatonin content in skin, at pre-véraison, whereas, at véraison, the highest levels were reported in the seed. Furthermore, during ripening, melatonin decreased in skin, while increasing in both seed and flesh. The relative concentrations of melatonin in diverse berry tissues were somewhat different from those of total polyphenols (TP), the latter measured by the Folin-Ciocalteau assay, and more abundant in seed at pre-véraison and in exocarp at véraison. The highest antiradical activity, determined by both DPPH (2,2-diphenyl-1-pycryl hydrazyl) and ABTS [(2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical-scavenging assay, was reported at pre-veráison in seed. To the best of our knowledge, we reported, for the first time, the occurrence of melatonin in grape seeds.

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