Therapeutic benefit of melatonin in experimental feline uveitis

Authors

  • María José Del Sole,

    1. Laboratory of Nervous System Physiology and Endocrinology, Department of Physiopathology, School of Veterinary Science, National University of the Center of Buenos Aires Province, Tandil
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  • Pablo H. Sande,

    1. Laboratory of Retinal Neurochemistry and Experimantal Ophthalmology, Department of Human Biochemistry, School of Medicine, University of Buenos Aires, CEFyBO/CONICET, Buenos Aires, Argentina
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  • Diego C. Fernandez,

    1. Laboratory of Retinal Neurochemistry and Experimantal Ophthalmology, Department of Human Biochemistry, School of Medicine, University of Buenos Aires, CEFyBO/CONICET, Buenos Aires, Argentina
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  • María Inés Keller Sarmiento,

    1. Laboratory of Retinal Neurochemistry and Experimantal Ophthalmology, Department of Human Biochemistry, School of Medicine, University of Buenos Aires, CEFyBO/CONICET, Buenos Aires, Argentina
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  • Marcelo A. Aba,

    1. Laboratory of Nervous System Physiology and Endocrinology, Department of Physiopathology, School of Veterinary Science, National University of the Center of Buenos Aires Province, Tandil
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  • Ruth E. Rosenstein

    1. Laboratory of Retinal Neurochemistry and Experimantal Ophthalmology, Department of Human Biochemistry, School of Medicine, University of Buenos Aires, CEFyBO/CONICET, Buenos Aires, Argentina
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Address reprint requests to Ruth E. Rosenstein, Departamento de Bioquímica Humana, Facultad de Medicina, CEFyBO, UBA, Paraguay 2155, 5º P, (1121), Buenos Aires, Argentina.
E-mail: ruthr@fmed.uba.ar

Abstract

Abstract:  Uveitis is a frequent ophthalmic disorder which constitutes one of the main causes of blindness in domestic cats. The aim of this report was to analyze the effect of melatonin on experimentally induced uveitis in cats. Bacterial lipopolysaccharide (LPS) was injected intravitreally into one eye from intact cats, while the contralateral eye was injected with vehicle. Melatonin was orally administered every 24 hr to a group of ten cats, from 24 hr before until 45 days after intravitreal injections. Eyes were evaluated by means of clinical evaluation, intraocular pressure (IOP), blood–ocular barrier integrity (via measurement of protein concentration and cell content in samples of aqueous humor [AH]), electroretinogram (ERG), and histological examination of the retinas. In LPS-treated eyes, several clinical signs were observed until day 45 postinjection. The treatment with melatonin significantly decreased clinical signs and prevented the reduction in IOP induced by LPS. In LPS-injected eyes, melatonin significantly preserved the blood–ocular barrier integrity, as shown by a decrease in the number of infiltrating cells and protein concentration in the AH. Mean amplitudes of scotopic ERG a- and b-waves were significantly reduced in eyes injected with LPS, whereas melatonin significantly prevented the effect of LPS. At 45 days after injection, LPS induced alterations in photoreceptors and at the middle portion of the retina, whereas melatonin preserved the retinal structure. These results indicate that melatonin prevented clinical, biochemical, functional, and histological alterations induced by LPS injection. Thus, melatonin might constitute a useful tool for the treatment of feline uveitis.

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