These authors contributed equally to this work.
Melatonin promotes embryonic development and reduces reactive oxygen species in vitrified mouse 2-cell embryos
Article first published online: 8 JAN 2012
© 2011 John Wiley & Sons A/S
Journal of Pineal Research
Volume 52, Issue 3, pages 305–311, April 2012
How to Cite
Gao, C., Han, H.-B., Tian, X.-Z., Tan, D.-X., Wang, L., Zhou, G.-B., Zhu, S.-E. and Liu, G.-S. (2012), Melatonin promotes embryonic development and reduces reactive oxygen species in vitrified mouse 2-cell embryos. Journal of Pineal Research, 52: 305–311. doi: 10.1111/j.1600-079X.2011.00944.x
- Issue published online: 20 MAR 2012
- Article first published online: 8 JAN 2012
- Accepted manuscript online: 29 NOV 2011 12:11AM EST
- Received October 7, 2011; Accepted November 23, 2011.
- embryonic development;
- mouse 2-cell embryos;
- reactive oxygen species;
Abstract: Two-cell embryos of mouse were vitrified by the open-pulled straw (OPS) method. The vitrified embryos were warmed and introduced into M16 medium for culture that contains melatonin at different concentrations (10−3, 10−5, 10−7, 10−9, 10−11 m). This process caused reactive oxygen species (ROS) formation and jeopardized the development of the embryos. Melatonin, at different concentrations, significantly suppresses ROS production and promotes embryonic development in vitrified embryos compared with untreated ones. The mechanistic studies indicated that the beneficial effects of melatonin on vitrified 2-cell embryos of mouse were melatonin receptor (MT1 and MT2) independent. The direct free radical scavenging activity, the enhancement of endogenous glutathione levels, and the anti-apoptotic capacity of melatonin may account for its protective effects on vitrified embryonic development.