Melatonin suppresses migration and invasion via inhibition of oxidative stress pathway in glioma cells
Article first published online: 8 MAR 2012
© 2012 John Wiley & Sons A/S
Journal of Pineal Research
Volume 53, Issue 2, pages 180–187, September 2012
How to Cite
Wang, J., Hao, H., Yao, L., Zhang, X., Zhao, S., Ling, E.-A., Hao, A. and Li, G. (2012), Melatonin suppresses migration and invasion via inhibition of oxidative stress pathway in glioma cells. Journal of Pineal Research, 53: 180–187. doi: 10.1111/j.1600-079X.2012.00985.x
- Issue published online: 13 AUG 2012
- Article first published online: 8 MAR 2012
- Accepted manuscript online: 9 FEB 2012 12:53PM EST
- Received December 8, 2011; Accepted February 1, 2012.
Abstract: Melatonin, an indolamine produced and secreted predominately by the pineal gland, exhibits a variety of physiological functions, possesses antioxidant and antitumor properties. In this study, we have shown that pharmacologic concentration (1 mm) of melatonin significantly reduced cell migration and invasion of T98G and U251 glioma cells after 24-hr treatment and inhibited expression of matrix metalloproteinase 2 (MMP 2) and MMP 9. The melatonin inhibition of cell migration and invasion was associated with its reduction of intracellular basal free radical generation. Melatonin at pharmacologic concentration also inhibited the constitutive activation of the reactive oxygen species downstream transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Furthermore, pyrrolidine dithiocarbamate, a NF-κB-specific inhibitor, at 10 μm displayed anti-migration and invasion effects and inhibition of MMP 2 and MMP 9 expression resembling that of melatonin. Taken together, it is concluded that inhibition of migration and invasion of glioma cells by melatonin is associated with the latter in its inhibition of oxidative stress pathway. This suggests a potential therapeutic application of melatonin in the treatment of glioma.