Deterium (2H) accumulation in the circulation was determined in 10 subjects after they ingested 7 ml · kg−1 of a dilute glucose-electrolyte solution that contained 10 g of 2H2O and was hot (50°C) on one occasion and cold (4°C) on another. In addition, 7 subjects repeated their first trial on a third occasion. Subjects fasted overnight and remained seated at rest during the experiment Blood 2H concentration was significantly greater at 5 min after ingestion of the hot as compared with the cold treatment and at 60 min for the cold as compared with the hot treatment The rate of blood 2H accumulation was not different when the same solution was ingested on different days. The results indicate that the rate of 2H accumulation is: 1) similar for hot and cold solutions and 2) reproducible when the same solution is ingested on different days. This implies that the rate at which dilute glucose-electrolyte solutions are made available to the body is not much influenced by the temperature at which they are ingested.