Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates
Version of Record online: 31 MAR 2005
Skin Research and Technology
Volume 11, Issue 2, pages 97–101, May 2005
How to Cite
Dreher, F., Modjtahedi, B. S., Modjtahedi, S. P. and Maibach, H. I. (2005), Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates. Skin Research and Technology, 11: 97–101. doi: 10.1111/j.1600-0846.2005.00103.x
- Issue online: 31 MAR 2005
- Version of Record online: 31 MAR 2005
- Accepted for publication 17 July 2004
- Lowry assay;
- homogeneity of removal;
- drug discovery
Background/aims: Examination of stratum corneum (SC) content with tape stripping and a colorimetric method is increasingly used. We examined the possible use of microplates in tandem with a colorimetric method to examine SC removed with tape stripping. As a corollary to this examination, the homogeneity of tape strips was examined.
Method: The commonly used Lowry assay was adapted for 96-well plates. Tapes were divided into four regions and sample disks of 5 mm diameter were taken from each and analyzed for SC mass using the adapted Lowry assay.
Results: Homogeneity of SC removal over different areas across a tape strip is limited.
Conclusion: Quantification of SC by means of a 96-well microplate-based colorimetric method is feasible and shortens the time of analysis. However, when using D-Squame® tape disks, SC removal on a limited area of the tape is not predictive for SC removal on the entire tape as removal is inhomogenous. Therefore, SC protein extraction should be performed on a large enough area, eventually on the entire tape when quantifying SC mass removed by tape stripping.