A new technique to in vivo study the corneocyte features at the surface of the skin
Article first published online: 19 MAR 2008
© 2008 L'Oréal. Journal compilation © 2008 Blackwell Munksgaard
Skin Research and Technology
Volume 14, Issue 4, pages 468–471, November 2008
How to Cite
Lévêque, J. L., François, G., Sojic, N. and Giron, F. (2008), A new technique to in vivo study the corneocyte features at the surface of the skin. Skin Research and Technology, 14: 468–471. doi: 10.1111/j.1600-0846.2008.00315.x
- Issue published online: 10 OCT 2008
- Article first published online: 19 MAR 2008
- Accepted for publication 30 December 2007
- corneocytes size;
- stratum corneum;
Purpose of the study: To design, develop and validate a new device allowing the features of corneocytes at the surface of the skin to be visualized in vivo and objectively characterized.
Materials and methods: Monochromatic light is focused at the proximal end of a coherent bundle of optical fibres. Fluorescence of skin, stained with fluorescein, is then captured by the same fibre bundle and displayed, through a dichroic mirror, by a CCD camera. Fluorescence images are analysed using dedicated software to measure the projected area of cells.
Results: The new device allows the mean projected area of corneocytes to be routinely studied and quantified on most of the skin areas of the human body. Measurements carried out on two age groups of women confirm that corneocyte size on the forearm is smaller in young women than in older women.
Conclusion: The new non-invasive device is easy to use and appear quite appropriate for cutaneous investigations carried out in clinical research.