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Use of Raman spectroscopy for the early detection of filaggrin-related atopic dermatitis

Authors


Address:
F. J. González
Coordinación para la Innovación y la Aplicación de la Ciencia y la Tecnología, Universidad Autónoma de San Luis Potosí,
Sierra Leona 550, Lomas 2da. Sección, 78210,
San Luís Potosí, SLP México
Tel: +52 444 825 0183 ext 232
Fax: +52 444 825 0198
e-mail: javier.gonzalez@uaslp.mx

Abstract

Background: Filaggrin (FLG) gene mutations, which result in complete or incomplete loss of proFLG/FLG peptides, have been reported as an important predisposing factor for atopic dermatitis (AD) and secondary atopic phenotypes such as atopic asthma.

Method: The presence of the protein FLG in the skin was evaluated at birth on 12 infants using Raman spectroscopy; these 12 infants were monitored for 1 year to see whether they developed AD. Three different statistical analysis procedures, two of which involved principal component analysis (PCA), were performed on the Raman spectra in order to determine the FLG content.

Results: The infants who had a lower FLG content, determined using any of the three statistical analysis procedures proposed, were also the ones that clinically developed AD.

Conclusion: The results suggest that Raman spectroscopy and statistical analysis such as PCA could be used as an early detection procedure for FLG -related AD and as a possible quantitative marker for FLG gene mutations.

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