Development and characterization of a human dermal equivalent with physiological mechanical properties

Authors

  • Gwenaël Rolin,

    1. Inserm UMR 645 Engineering and Cutaneous Biology Team, Besançon, France
    2. University of Franche-Comté, Besançon, France
    3. Clinical Investigation Center (CIC-BT 506 and CIC-IT 808), University Hospital of Besançon, Besançon, France
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  • Vincent Placet,

    1. University of Franche-Comté, Besançon, France
    2. Department of Applied Mechanics, FEMTO-ST Institute, Besançon, France
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  • Emmanuelle Jacquet,

    1. University of Franche-Comté, Besançon, France
    2. Department of Applied Mechanics, FEMTO-ST Institute, Besançon, France
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  • Hélène Tauzin,

    1. Inserm UMR 645 Engineering and Cutaneous Biology Team, Besançon, France
    2. University of Franche-Comté, Besançon, France
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  • Sophie Robin,

    1. Bioexigence, Besançon, France
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  • Lionel Pazart,

    1. Clinical Investigation Center (CIC-BT 506 and CIC-IT 808), University Hospital of Besançon, Besançon, France
    2. University Hospital of Besançon, Besançon, France
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  • Celine Viennet,

    1. Inserm UMR 645 Engineering and Cutaneous Biology Team, Besançon, France
    2. University of Franche-Comté, Besançon, France
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  • Philippe Saas,

    1. Inserm UMR 645 Engineering and Cutaneous Biology Team, Besançon, France
    2. University of Franche-Comté, Besançon, France
    3. Clinical Investigation Center (CIC-BT 506 and CIC-IT 808), University Hospital of Besançon, Besançon, France
    4. EFS Bourgogne Franche-Comté, Besançon, France
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  • Patrice Muret,

    1. Inserm UMR 645 Engineering and Cutaneous Biology Team, Besançon, France
    2. University Hospital of Besançon, Besançon, France
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  • Delphine Binda,

    1. Inserm UMR 645 Engineering and Cutaneous Biology Team, Besançon, France
    2. Clinical Investigation Center (CIC-BT 506 and CIC-IT 808), University Hospital of Besançon, Besançon, France
    3. University Hospital of Besançon, Besançon, France
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  • Philippe Humbert

    Corresponding author
    1. University of Franche-Comté, Besançon, France
    2. University Hospital of Besançon, Besançon, France
    • Inserm UMR 645 Engineering and Cutaneous Biology Team, Besançon, France
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Address:

Pr. P. Humbert

Department of Dermatology

University Hospital of Besançon

2 Place St Jacques

25030 Besançon Cedex

France

Tel: +33(3) 81 21 83 01

Fax: +33(3) 81 21 82 79

e-mail: philippe.humbert@univ-fcomte.fr

Abstract

Background/aims

Different models of reconstructed skin are available, either to provide skin wound healing when this process is deficient, or to be used as an in vitro model. Nevertheless, few studies have focused on the mechanical properties of skin equivalent. Indeed, human skin is naturally under tension. Taking into account these features, the purpose of this work was to obtain a cellularized dermal equivalent (CDE), composed of collagen and dermal fibroblasts.

Methods

To counteract the natural retraction of CDE and to maintain it under tension, different biomaterials were tested. Selection criteria were biocompatibility, bioadhesion properties, ability to induce differentiation of fibroblasts into myofibroblasts and mechanical characterization, considering that of skin in vivo. These assays led to the selection of honeycomb of polyester. CDE constructed on this biomaterial was further characterized mechanically using tensile tests.

Results

The results showed that mechanical features of the obtained dermal equivalent, including myofibroblasts, were similar to skin in vivo.

Conclusion

The original model of dermal equivalent presented herein may be a useful tool for clinical use and as an in vitro model for toxicological/pharmacological research.

Ancillary