Get access

Sulfur mustard cutaneous injury characterization based on SKH-1 mouse model: relevance of non-invasive methods in terms of wound healing process analyses

Authors

  • Cécile Cléry-Barraud,

    Corresponding author
    • Chemical Burn Unit, Toxicology and Chemical Risk Department, Armed Forces Biomedical Research Institute IRBA, 38702 La Tronche Cedex, France
    Search for more papers by this author
  • Nina Nguon,

    1. Chemical Burn Unit, Toxicology and Chemical Risk Department, Armed Forces Biomedical Research Institute IRBA, 38702 La Tronche Cedex, France
    Search for more papers by this author
  • Virginie Vallet,

    1. Chemical Burn Unit, Toxicology and Chemical Risk Department, Armed Forces Biomedical Research Institute IRBA, 38702 La Tronche Cedex, France
    Search for more papers by this author
  • Catherine Sentenac,

    1. Chemical Burn Unit, Toxicology and Chemical Risk Department, Armed Forces Biomedical Research Institute IRBA, 38702 La Tronche Cedex, France
    Search for more papers by this author
  • Elise Four,

    1. Chemical Burn Unit, Toxicology and Chemical Risk Department, Armed Forces Biomedical Research Institute IRBA, 38702 La Tronche Cedex, France
    Search for more papers by this author
  • Carine Arlaud,

    1. Chemical Burn Unit, Toxicology and Chemical Risk Department, Armed Forces Biomedical Research Institute IRBA, 38702 La Tronche Cedex, France
    Search for more papers by this author
  • David Coulon,

    1. Animal Husbandy Service, Toxicology and Chemical Risk Department, Armed Forces Biomedical Research Institute IRBA, La Tronche Cedex, France
    Search for more papers by this author
  • Isabelle Boudry

    1. Chemical Burn Unit, Toxicology and Chemical Risk Department, Armed Forces Biomedical Research Institute IRBA, 38702 La Tronche Cedex, France
    Search for more papers by this author

Address:

Cécile Cléry-Barraud

Chemical Burn Unit

Toxicology and Chemical Risk Department

Armed Forces Biomedical Research Institute IRBA

24 Avenue des Maquis du Grésivaudan

38702 La Tronche Cedex

France

Tel: +33 4 76639740

Fax: +33 4 76636962

e-mail: cecile.clerybarraud@sfr.fr or cclerybarraud@crssa.net

Abstract

Background

To date, sulphur mustard (SM) cutaneous toxicity has been commonly assessed on account of several animal models such as pigs and weanling pigs. Few experiments however, have been carried out on mice so far. In this study, we aimed at quantifying spontaneous wound healing processes after SM exposure on a SKH-1 mouse model through non-invasive methods over an extended period of time.

Methods

Animals were exposed to 10 μL net SM in a vapor cup system. Measurements of barrier function (Transepidermal water loss), elasticity, skin color exposed to SM vapors were determined by evaporimetry, cutometer and image analysis on 23 animals up to 28 days. Results were subsequently correlated with histological and biochemical analyses.

Results

The TEWL parameter stands as a top-ranking criterion to keep track of skin barrier restoration after SM cutaneous intoxication in our SKH-1 mouse model. The R2 and R6 elasticity parameters or for the skin color exhibited their ability to be restored after 28 days of SM exposure.

Conclusion

Our findings suggest that bio-engineering methods are eligible to evaluate new treatments on SM-induced skin SKH-1 mouse lesions, thus making an allowance for less invasive methods such as histological, genomic or proteomic approaches.

Get access to the full text of this article

Ancillary