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Terahertz wave techniques using a metal mesh for evaluating the components of the stratum corneum

Authors


Address:

Koji Mizukoshi

POLA Chemical Industries, Inc

560 Kashio-cho

Totsuka-ku

Yokohama

224-0812

Japan

Tel: +81 45 826 7134

Fax: +81 45 826 7239

e-mail : k-mizukoshi@pola.co.jp

Abstract

Background/purpose

Terahertz waves are located in the region of the spectrum between milliwaves and infrared. We analyzed the feasibility of terahertz spectroscopy to inspect the compositional variations of the stratum corneum (SC).

Methods

We used a terahertz time-domain spectroscopy system with the metal mesh technique. To investigate whether terahertz can inspect compositional variation of SC, we measured the terahertz frequency spectra of the SC sheet that was treated with chloroform–methanol, lipid mixture, a denaturation agent, and heating with hot air.

Results

The chloroform–methanol treatment of the SC shifted the dip position, which represents a convex downward shape of the spectra, to a higher frequency. This dip shift was reversed to an untreated position by the dose-dependent application of a lipid mixture. The heating treatment of the SC shifted the dip position to a higher frequency. The same dip shift was also induced by the application of a denaturation agent to the SC.

Conclusion

The technique using terahertz waves with a metal mesh is effective because of its simplicity and its high degree of accuracy in detecting the amount of lipid and the protein conformation state.

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