Influence of surface charge of gold nanorods on skin penetration
Article first published online: 2 JUL 2012
© 2012 John Wiley & Sons A/S
Skin Research and Technology
Volume 19, Issue 1, pages e390–e396, February 2013
How to Cite
Lee, O., Jeong, S. H., Shin, W. U., Lee, G., Oh, C. and Son, S. W. (2013), Influence of surface charge of gold nanorods on skin penetration. Skin Research and Technology, 19: e390–e396. doi: 10.1111/j.1600-0846.2012.00656.x
- Issue published online: 7 JAN 2013
- Article first published online: 2 JUL 2012
- Manuscript Accepted: 26 APR 2012
- skin penetration;
- human skin equivalent model;
- gold nanorods;
- surface charge;
The skin plays an important role as a protective barrier against toxic environments and also is a route of drug administration. In spite of evidence for and interest in the skin penetration of nanoparticles, no study has examined the effect of nanoparticle surface charge on percutaneous absorption. In this study, we investigated the effect of surface charges of gold nanorods (GNs) on skin penetration.
Using transmission electron microscopy (TEM) and image analysis, we quantitatively measured the ability of GNs to penetrate the skin.
Our results showed that the area density of the electron-dense dots of GNs, which penetrated into the stratum corneum, significantly increased for negatively charged GNs compared to those with a positive charge (P < 0.01). To investigate the percutanoues absorption of charged GNs, in vitro skin permeation studies were carried out using a Franz-type diffusion cell (FDC). The penetration of GNs through the skin was quantified by inductively coupled plasma mass spectrometry. Consistent with TEM observations, our penetration study using an FDC also revealed that negative particles were frequently detected in samples of receptor fluid at 48 h after exposure (P < 0.01).
Together our results showed that anionic GNs penetrate skin better than cationic GNs.