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Influence of surface charge of gold nanorods on skin penetration

Authors

  • Onseok Lee,

    1. Department of Radiological Science, College of Natural Science, Gimcheon University, Gimcheon City, Gyeangbuk, Korea
    2. Institute for Health Science, Gimcheon University, Gimcheon City, Gyeangbuk, Korea
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    • Contributed equally.
  • Sang Hoon Jeong,

    1. Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology, Korea University College of Medicine, Seoul, Korea
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    • Contributed equally.
  • Won Ung Shin,

    1. Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology, Korea University College of Medicine, Seoul, Korea
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  • Gunwoo Lee,

    1. Division of the Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea
    2. Research Institute for Skin Image, Korea University Medical Center, Seoul, Korea
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  • Chilhwan Oh,

    1. Division of the Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea
    2. Research Institute for Skin Image, Korea University Medical Center, Seoul, Korea
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  • Sang Wook Son

    Corresponding author
    1. Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology, Korea University College of Medicine, Seoul, Korea
    2. Division of the Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea
    • Department of Radiological Science, College of Natural Science, Gimcheon University, Gimcheon City, Gyeangbuk, Korea
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Address:

Sang Wook Son

Department of Dermatology

Korea University Anam Hospital

Korea University College of Medicine

126-1, Anam-dong 5-ga

Seongbuk-gu

Seoul 136-705

Korea

Tel: +82-2-920-6433

Fax: +82-2-928-7540

e-mail: skin4u@korea.ac.kr

Abstract

Background/purpose

The skin plays an important role as a protective barrier against toxic environments and also is a route of drug administration. In spite of evidence for and interest in the skin penetration of nanoparticles, no study has examined the effect of nanoparticle surface charge on percutaneous absorption. In this study, we investigated the effect of surface charges of gold nanorods (GNs) on skin penetration.

Methods

Using transmission electron microscopy (TEM) and image analysis, we quantitatively measured the ability of GNs to penetrate the skin.

Results

Our results showed that the area density of the electron-dense dots of GNs, which penetrated into the stratum corneum, significantly increased for negatively charged GNs compared to those with a positive charge (P < 0.01). To investigate the percutanoues absorption of charged GNs, in vitro skin permeation studies were carried out using a Franz-type diffusion cell (FDC). The penetration of GNs through the skin was quantified by inductively coupled plasma mass spectrometry. Consistent with TEM observations, our penetration study using an FDC also revealed that negative particles were frequently detected in samples of receptor fluid at 48 h after exposure (P < 0.01).

Conclusion

Together our results showed that anionic GNs penetrate skin better than cationic GNs.

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