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Dynamic Organization of the Actin Cytoskeleton During Meiosis and Spore Formation in Budding Yeast
Article first published online: 14 NOV 2006
DOI: 10.1111/j.1600-0854.2006.00496.x
2006 Blackwell Publishing Ltd
Additional Information
How to Cite
Taxis, C., Maeder, C., Reber, S., Rathfelder, N., Miura, K., Greger, K., Stelzer, E. H. K. and Knop, M. (2006), Dynamic Organization of the Actin Cytoskeleton During Meiosis and Spore Formation in Budding Yeast. Traffic, 7: 1628–1642. doi: 10.1111/j.1600-0854.2006.00496.x
Publication History
- Issue published online: 14 NOV 2006
- Article first published online: 14 NOV 2006
- Received 3 March 2006; revised and accepted for publication 6 September 2006
Keywords:
- actin;
- cell polarity;
- meiosis;
- sporulation;
- vesicle movement;
- yeast
During sporulation in Saccharomyces cerevisiae, the four daughter cells (spores) are formed inside the boundaries of the mother cell. Here, we investigated the dynamics of spore assembly and the actin cytoskeleton during this process, as well as the requirements for filamentous actin during the different steps of spore formation. We found no evidence for a polarized actin cytoskeleton during sporulation. Instead, a highly dynamic network of non-polarized actin cables is present underneath the plasma membrane of the mother cell. We found that a fraction of prospore membrane (PSM) precursors are transported along the actin cables. The velocity of PSM precursors is diminished if Myo2p or Tpm1/2p function is impaired. Filamentous actin is not essential for meiotic progression, for shaping of the PSMs or for post-meiotic cytokinesis. However, actin is essential for spore wall formation. This requires the function of the Arp2/3p complex and involves large carbohydrate-rich compartments, which may be chitosome analogous structures.