These authors contributed equally to this manuscript.
Reassessing the Role of Phosphocaveolin-1 in Cell Adhesion and Migration
Article first published online: 14 SEP 2007
2007 Blackwell Publishing Ltd
Volume 8, Issue 12, pages 1695–1705, December 2007
How to Cite
Hill, M. M., Scherbakov, N., Schiefermeier, N., Baran, J., Hancock, J. F., Huber, L. A., Parton, R. G. and Parat, M.-O. (2007), Reassessing the Role of Phosphocaveolin-1 in Cell Adhesion and Migration. Traffic, 8: 1695–1705. doi: 10.1111/j.1600-0854.2007.00653.x
- Issue published online: 14 SEP 2007
- Article first published online: 14 SEP 2007
- Received 18 August 2007, revised and accepted for publication 10 September 2007, uncorrected manuscript published online 14 September 2007
- cell adhesion;
- cell migration;
- focal adhesions;
- tyrosine phosphorylation
Although phosphorylation on tyrosine 14 was identified early in the discovery of caveolin-1, the functional significance of this modification still remains elusive. Recent evidence points to a role of caveolin-1 tyrosine 14 phosphorylation in cell adhesion and migration. These results are based on a variety of tools, including a widely used mouse monoclonal anti-phosphocaveolin-1 antibody, which labels, in cultured cells, a protein localized at or near focal adhesions. We here report results from three independent laboratories, showing that this antibody recognizes phosphocaveolin-1 amongst other proteins in immunoblot analyses and that the signal obtained with this antibody in immunostaining experiments is in part due to labeling of paxillin. Published data need to be interpreted keeping in mind that images of phosphocaveolin-1 cellular localization obtained using this antibody are not valid. We re-evaluate the current knowledge about the role of caveolin-1 in cell adhesion and migration in view of this new information.