These authors contributed equally to this work.
Overexpression of a Plant Reticulon Remodels the Lumen of the Cortical Endoplasmic Reticulum but Does not Perturb Protein Transport
Article first published online: 2 NOV 2007
2007 Blackwell Publishing Ltd
Volume 9, Issue 1, pages 94–102, January 2008
How to Cite
Tolley, N., Sparkes, I. A., Hunter, P. R., Craddock, C. P., Nuttall, J., Roberts, L. M., Hawes, C., Pedrazzini, E. and Frigerio, L. (2008), Overexpression of a Plant Reticulon Remodels the Lumen of the Cortical Endoplasmic Reticulum but Does not Perturb Protein Transport. Traffic, 9: 94–102. doi: 10.1111/j.1600-0854.2007.00670.x
- Issue published online: 2 NOV 2007
- Article first published online: 2 NOV 2007
- Received 5 June 2007, revised and accepted for publication 29 October 2007, uncorrected manuscript published online 2 November 2007
- Arabidopsis thaliana;
- endoplasmic reticulum;
- organelle shape;
- protein transport;
- secretory pathway
We have cloned a member of the reticulon (RTN) family of Arabidopsis thaliana (RTNLB13). When fused to yellow fluorescent protein (YFP) and expressed in tobacco leaf epidermal cells, RTNLB13 is localized in the endoplasmic reticulum (ER). Coexpression of a soluble ER luminal marker reveals that YFP-tagged, myc-tagged or untagged RTNLB13 induces severe morphological changes to the lumen of the ER. We show, using fluorescence recovery after photobleaching (FRAP) analysis, that RTNLB13 overexpression greatly reduces diffusion of soluble proteins within the ER lumen, possibly by introducing constrictions into the membrane. In spite of this severe phenotype, Golgi shape, number and dynamics appear unperturbed and secretion of a reporter protein remains unaffected.