Trafficking of Vesicular Neurotransmitter Transporters

Authors

  • Hao Fei,

    1. 1 Gonda (Goldschmied) Neuroscience and Genetics Research Center, Departments of Psychiatry and
      #Neurobiology, David Geffen School of Medicine at UCLA, 695 Charles Young Drive South, Los Angeles, CA 90095-1761, USA
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  • 1 Anna Grygoruk,

    1. 1 Gonda (Goldschmied) Neuroscience and Genetics Research Center, Departments of Psychiatry and
      #Neurobiology, David Geffen School of Medicine at UCLA, 695 Charles Young Drive South, Los Angeles, CA 90095-1761, USA
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  • 1 Elizabeth S. Brooks,

    1. 1 Gonda (Goldschmied) Neuroscience and Genetics Research Center, Departments of Psychiatry and
      #Neurobiology, David Geffen School of Medicine at UCLA, 695 Charles Young Drive South, Los Angeles, CA 90095-1761, USA
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  • 1 Audrey Chen,

    1. 1 Gonda (Goldschmied) Neuroscience and Genetics Research Center, Departments of Psychiatry and
      #Neurobiology, David Geffen School of Medicine at UCLA, 695 Charles Young Drive South, Los Angeles, CA 90095-1761, USA
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  • and 1, David E. Krantz 1,

    Corresponding author
    1. 1 Gonda (Goldschmied) Neuroscience and Genetics Research Center, Departments of Psychiatry and
      #Neurobiology, David Geffen School of Medicine at UCLA, 695 Charles Young Drive South, Los Angeles, CA 90095-1761, USA
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*David E. Krantz, dkrantz@ucla.edu

Abstract

Vesicular neurotransmitter transporters are required for the storage of all classical and amino acid neurotransmitters in secretory vesicles. Transporter expression can influence neurotransmitter storage and release, and trafficking targets the transporters to different types of secretory vesicles. Vesicular transporters traffic to synaptic vesicles (SVs) as well as large dense core vesicles and are recycled to SVs at the nerve terminal. Some of the intrinsic signals for these trafficking events have been defined and include a dileucine motif present in multiple transporter subtypes, an acidic cluster in the neural isoform of the vesicular monoamine transporter (VMAT) 2 and a polyproline motif in the vesicular glutamate transporter (VGLUT) 1. The sorting of VMAT2 and the vesicular acetylcholine transporter to secretory vesicles is regulated by phosphorylation. In addition, VGLUT1 uses alternative endocytic pathways for recycling back to SVs following exocytosis. Regulation of these sorting events has the potential to influence synaptic transmission and behavior.

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