The first two authors equally contributed to this paper.
Protein Kinase D-Dependent Trafficking of the Large Herpes simplex Virus Type 1 Capsids from the TGN to Plasma Membrane
Version of Record online: 5 MAY 2009
© 2009 John Wiley & Sons A/S
Volume 10, Issue 8, pages 1074–1083, August 2009
How to Cite
Rémillard-Labrosse, G., Mihai, C., Duron, J., Guay, G. and Lippé, R. (2009), Protein Kinase D-Dependent Trafficking of the Large Herpes simplex Virus Type 1 Capsids from the TGN to Plasma Membrane. Traffic, 10: 1074–1083. doi: 10.1111/j.1600-0854.2009.00939.x
- Issue online: 2 JUL 2009
- Version of Record online: 5 MAY 2009
- Received 19 November 2008, revised and accepted for publication 1 May 2009, uncorrected manuscript published online 5 May 2009, published online 18 June 2009
- intracellular transport;
- large cargo;
- serine-threonine protein kinase;
- trans Golgi network;
The biosynthetic pathway carries cargos from the endoplasmic reticulum (ER) to the trans Golgi network (TGN) via a typical passage through the Golgi. Interestingly, large particles such as procollagen, chylomicrons and some viruses all reach the TGN by atypical routes. Given this dichotomy, we anticipated that such cargos might rely on non-classical machineries downstream of the TGN. Using Herpes simplex virus type 1 (HSV-1) as a model and a synchronized infection protocol that focuses on TGN to plasma membrane transport, the present study revealed the surprising implication of the cellular serine-threonine protein kinase D in HSV-1 egress. These findings, confirmed by a variety of complementary means [pharmacological inhibitors, dominant negative mutant, RNA interference and electron microscopy (EM)], identify one of possibly several cellular factors that modulate the egress of viruses transiting at the TGN. Moreover, the involvement of this kinase, previously known to regulate the transport of small basolateral cargos, highlights the trafficking of both small and exceptionally large entities by a common machinery downstream of the TGN, in sharp contrast to earlier steps of transport. Conceptually, this indicates the TGN is not only a sorting station from which cargos can depart towards different destinations but also a meeting point where conventional and unconventional routes can meet along the biosynthetic pathway. Lastly, given the apical release of HSV-1 in neurons, it opens up the possibility that this kinase might regulate some apical sorting.